DNA2

Question 1

What is the precursor molecule for purines?

Answer 1

inosine

Question 2

What is the final product of purines?

Answer 2

Uric Acid

Question 3

Draw Uric Acid

Answer 3

See Slide 8 DNA2

Question 4

Uric acid is highly active due to what two things:

Answer 4

lots of donors and lots of acceptors

Question 5

AMP synthesis depends on …..

Answer 5

GTP

Question 6

….. synthesis depends on ATP

Answer 6

GMP

Question 7

Furanose rings can be usefully substituted with what?

Answer 7

phosphates

Question 8

What functional groups on the furanose ring can be substituted to create polymers?

Answer 8

-OH

Question 9

Which carbons on the furanose rings are linkage sites for polymer synthesis?

Answer 9

5’C and 3’C

Question 10

What attributes to the flexibility and number of conformations a base has?

Answer 10

rotatable bonds

Question 11

The ….. bond can change between syn and anti.

Answer 11

x (chi) dihedral, glycosidic linkage, bond between sugar and base;

Question 12

Draw syn-adenosine

Answer 12

Slide 11 DNA2

Question 13

Draw anti-adenosine

Answer 13

Slide 11 DNA2

Question 14

When A/TU and G/C pair up with their H-bonds, what carbons give similar distances?

Answer 14

C-1’ to C-1’
Slide 13 DNA2

Question 15

DNA and RNA polymer strands are ….. and have direction given by the sugar

Answer 15

asymmetric

Question 16

Draw the synthesis pathway of GTP and ATP, including pathway inhibitors and positive factors.

Answer 16

See slide 9 DNA2

Question 17

What is used to analytically detect the electronic differences in the rings and substitutions of bases?

Answer 17

UV absorption

Question 18

What is detected in UV absorption spectrums of nucelic acid bases?

Answer 18

electronic differences in the rings and substitutions

Question 19

What is the peak wavelength of GMP in UV absorption?

Answer 19

245nm

Question 20

What is the max wavelength of AMP in UV absorption?

Answer 20

260nm

Question 21

What is the max wavelength of UMP in UV absorption?

Answer 21

260nm

Question 22

What is the max wavelength of dTMP in UV absorption?

Answer 22

270nm

Question 23

What is the max wavelength of CMP in UV absorption?

Answer 23

275nm

Question 24

What exists due the several titratable groups of bases?

Answer 24

Tautomers (resonance)

Question 25

What factors allow for purine and pyrimidine tautomers to exist?

Answer 25

1. solvent polarity
2. pH
   (environment)

Question 26

Besides pH and titratable groups, what allows for resonance states of bases?

Answer 26

hetero atoms

Question 27

Which type of nucleic acid bases have more tautonomers?

Answer 27

pyrimidines

Question 28

If you received a number representing the U(r) of Coloumb’s law as: 0.0000001, what can you assume about the molecules in this solution?

Answer 28

Since the nnumber is close to zero, we can assume that it is unlikely these molecules will interact with each other.

Question 29

When counterion condensation increases does screening increase or decrease?

Answer 29

increase