DNA5 Flashcards

1
Q

In translation, where does recognition of tRNA happen?

A

A-site; decoding site

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2
Q

In translation, where does catalysis happen?

A

P-site; transferase center

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3
Q

As a whole unit, what ultracentrifuge weight does the translational rRNA have?

A

70s

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4
Q

What’s the ultracentrifugal weight of small unit of translational rRNA?

A

30S

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5
Q

What’s the ultracentrifugal weight of the large subunit of translational rRNA?

A

50S

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6
Q

What is the role of the 30S subunit during translation?

A

hold mRNA

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7
Q

what is the role of the 50S subunit in translation?

A

catalyze the peptide bond formation

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8
Q

Translocation and binding of tRNA during translation is dependent on what?

A

GTP energy source

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9
Q

The ….. ….. signals the end of protein synthesis.

A

release factor

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10
Q

what charges tRNA with the correct amino acid?

A

tRNA Synthetases

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11
Q

What marks an activated compound that is ready for transfer during translation?

A

formylation

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12
Q

What is the start sequence of translation?

A

formylated methionine

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13
Q

What reaction is catalyzed at the active site of the ribosome for translation?

A

lactam condensation

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14
Q

How many proteins are in the prokaryotic ribosome?

A

55

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15
Q

How many proteins are in eukaryotic ribosomes?

A

79-80

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16
Q

There are no …… in the peptidyl transferase of the ribosome

A

proteins

17
Q

What happens to the ribosome in cells when it stalls due to stress factors?

A

rescue factors (in bacteria) dissociate translationally inactive ribosomes into their subunits

18
Q

What are two recycling factors found in bacteria?

A

GTPase and HflXr

19
Q

What is ribosomal hibernation?

A

An inactive state in which ribosomes are dissociate and bind to ‘hibernation’ factors