DNA translation Flashcards
what is translation
the synthesis of a linear chain of amino acids (primary transcript)
what does translation require
aminoacyl-tRNA
ribosomes
mRNA
what is the site of translation
ribosomes
functional 80S ribsomoe is composed of what
2 subunits
what subunits are contained by a large 60S subunit
5S rRNA- 120 nucleotides
28S rRNA- 2,700 nucleotides
5.8S rRNA- 160 nucleotides
about 49 ribosomal proteins
what does a small 40S subunit contain
18S rRNA
about 33 ribosomal proteins
what fit together to form a cleft
the 2 irregularly shaped subunits (40S and 60S)
how do the mRNA and cleft interact
mRNA moves through the cleft as the ribosome passes along the mRNA during translation
where is mRNA bound
to the small 40S subunit
how many binding sites does the 60S subunit contain
2
what are the binding sites on the 60S subunit called
P site and A site
what is the purpose of the P site on the 60S subunit
growing polypeptide chain is bound to this site (peptidyl-tRNA site)
what is the purpose of the A site on the 60S subunit
new aminoacyl-tRNA to be added to growing polypeptide chain is bound at this site (aamino-tRNA site)
what does translation proceed from and to
N-terminus to C-terminus of protein
what direction is mRNA read in
5/ to 3/