DNA Structure and Replication Flashcards
1
Q
What base pairing is usually found at origin of replication sites? Why?
A
A-T. Because it only has two Hydrogen bonds rather than 3.
2
Q
What is the backbone of DNA?
A
Sugar-phosphate.
3
Q
In which direction does DNA Polymerase work?
A
3’ to 5’
4
Q
In which direction does DNA Polymerase build?
A
5’ to 3’
5
Q
What are two mechanisms that fix mistakes generated during DNA replication?
A
DNA Polymerase and DNA Repair Enzymes.
6
Q
What must be put into the PCR mixture for it to work?
A
Mix DNA template with primers, DNA building blocks, and DNA Polymerase.
7
Q
What are the 3 stages of PCR?
A
- Heat at 95C for 30 sec (denaturation).
- Cool to 60C for 30 sec (annealing).
- Heat to 72C for 60 sec (elongation).
8
Q
What are the substrates of DNA replication?
A
Primer (RNA), bases.
9
Q
What is DNA catalyzed by?
A
DNA polymerase.
