DNA replication And PCR

Question 1

DNA replication

Answer 1

Process that makes an exact copy of DNA

Question 2

DNA polymerase

Answer 2

Enzyme that adds free DNA nucleotides to the 3’ end strand of template stand

Question 3

Primer

Answer 3

A short complementary strand of nucleotides that bind to a target sequence allowing DNA polymerase to add DNA nucleotides

Question 4

ATP

Answer 4

Energy molecule required for replication of DNA

Question 5

Free nucleotides

Answer 5

Subunits of DNA That are required for DNA replication, added by DNA polymerase to the 3’ end of the growing strand

Question 6

Leading strand

Answer 6

Replicated continuously by DNA polymerase

Question 7

Lagging strand

Answer 7

Replicated in fragments by DNA polymerase

Question 8

Polymerase chain reaction

Answer 8

PCR

Technique used to amplify DNA

Question 9

Solving crimes

Answer 9

A use of PCR if a DNA sample is collected from a crime scene

Question 10

Paternity testing

Answer 10

A use of PCR in the identification of a father

Question 11

Genetic testing

Answer 11

A use of PCR to test for the presence or absence of a genetic disease

Question 12

Heat tolerant DNA polymerase

Answer 12

Enzyme used in PCR to add free nucleotides to the new strand

Question 13

Temperatures in PCR

Answer 13

95•C temperature heated to in order to break hydrogen bonds between bases

55•C temperature DNA is cooled to allowing primers to attach to target sequences

75•C temperature heated to allowing heat tolerant DNA polymerase to replicate