DNA recombination Flashcards

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1
Q

Define general recombination

A

switching that occurs between pairs of homologous sequences

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2
Q

Define RecA protein

A

used for synapsis of a single strand of DNA into a dsDNA

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3
Q

What are two homologues for RecA in eukaryotes that you should know??

A

Rad51 and Dmc1

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4
Q

the process of cutting the Holliday junction is called

A

resolution

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5
Q

What are the two enzymes that cut ds DNA in homologous recombination

A

Spo11 and Mre11 nuclease complex

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6
Q

What is a site of gene conversion?

A

where one strand was present in the middle of another gene

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7
Q

What is a Site of crossover?

A

Where heteroduplex forms between two joined strands of dsDNA

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8
Q

What is an example of DNA-only transposons?

A

P-element (Drosophila)

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9
Q

What is an example of a retroviral-like retrotransposon?

A

Ty1 (yeast)

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10
Q

What is an example of nonretroviral retrotransposons

A

L1 (human)

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11
Q

What enzyme is required for DNA transposons

A

transposase

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12
Q

What enzymes are required for retroviral-like retrotransposons

A

reverse transcriptase and integrase

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13
Q

What enzymes are required for nonretroviral retrotransposons

A

reverse transcriptase and endonuclease

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14
Q

Describe the ends of a DNA transposon

A

short inverted repeats at the end

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15
Q

Describe the ends of a retroviral-like retrotransposons

A

directly repeated long terminal repeats (LTRs) at each end

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16
Q

Describe the ends nonretroviral retrotransposons

A

Poly A tail at 3’ end of RNA transcript, 5’ end often shortened

17
Q

What is the difference between CRE and LoxP?

A

Cre is a recombinase protein. LoxP is the sequence Cre cuts at.