DNA recombination

Question 1

Define general recombination

Answer 1

switching that occurs between pairs of homologous sequences

Question 2

Define RecA protein

Answer 2

used for synapsis of a single strand of DNA into a dsDNA

Question 3

What are two homologues for RecA in eukaryotes that you should know??

Answer 3

Rad51 and Dmc1

Question 4

the process of cutting the Holliday junction is called

Answer 4

resolution

Question 5

What are the two enzymes that cut ds DNA in homologous recombination

Answer 5

Spo11 and Mre11 nuclease complex

Question 6

What is a site of gene conversion?

Answer 6

where one strand was present in the middle of another gene

Question 7

What is a Site of crossover?

Answer 7

Where heteroduplex forms between two joined strands of dsDNA

Question 8

What is an example of DNA-only transposons?

Answer 8

P-element (Drosophila)

Question 9

What is an example of a retroviral-like retrotransposon?

Answer 9

Ty1 (yeast)

Question 10

What is an example of nonretroviral retrotransposons

Answer 10

L1 (human)

Question 11

What enzyme is required for DNA transposons

Answer 11

transposase

Question 12

What enzymes are required for retroviral-like retrotransposons

Answer 12

reverse transcriptase and integrase

Question 13

What enzymes are required for nonretroviral retrotransposons

Answer 13

reverse transcriptase and endonuclease

Question 14

Describe the ends of a DNA transposon

Answer 14

short inverted repeats at the end

Question 15

Describe the ends of a retroviral-like retrotransposons

Answer 15

directly repeated long terminal repeats (LTRs) at each end

Question 16

Describe the ends nonretroviral retrotransposons

Answer 16

Poly A tail at 3’ end of RNA transcript, 5’ end often shortened

Question 17

What is the difference between CRE and LoxP?

Answer 17

Cre is a recombinase protein. LoxP is the sequence Cre cuts at.