DNA packaging Flashcards
How do prokaryotic cells divide
fission
fission
- they have one circular chromosome
- as DNA replicates it attaches to membrane
- new plasma membrane between attached points
- Cytokinesis separates the one cell into two
How do eukaryotes divide
mitosis , meiosis
constriction point of a chromosome
centromere
arms of chromosome
short - p arm
long - q arm
Chromatin
DNA + Histone + other proteins making up chromosomes
What energy is needed for coiling
Histones provide energy
What is a histone
positively charged protein, that DNA is compact chromosomal DNA
charge of DNA
negative due to phosphate groups in its phosphate sugar backbone
Nucleosome
repeating structural and functional unit of chromatin
- 9 histones, 166 base pairs
functions of chromatin
- package DNA
- strengthen DNA for division
- control gene expression and replication
Two forms chromatin is divided between
Heterochromatin (condensed)
Euchromatin (Extended)
Two mechanisms by which chromatin is made more accessible
- histones enzymatically modified
- histones displaced by chromatin remodelling complexes
= reversible
Prometaphase
Nuclear envelop breaks down
microtubules attach to chromosome
Metaphase
chromosomes line up in the centre on the metaphase plate
spindle fibres are attached