DNA cont.

Question 1

introns

Answer 1

noncoding regions of dna/rna

Question 2

exons

Answer 2

coding regions translated and expressed

Question 3

spliceosomes

Answer 3

cut out introns, joins exons

Question 4

what are added to the primary transcript to protect him digestion

Answer 4

5’ cap, 3’ poly a tail from poly a polymerase (made of adenine ribonucleotides)

Question 5

mutagens

Answer 5

chemicals/radiation that increase likelihood of a mutation occurring

Question 6

purines pair with pyrimidines to increase ______

Answer 6

stability

Question 7

bacterial plasmids

Answer 7

extra chromosomal dna in the cytoplasm of bacterial cells

Question 8

selection site

Answer 8

gene for antibiotic resistance

Question 9

endosymbiotic relationship

Answer 9

both benefit
- eg. bacteria and plasminds

Question 10

cloning region

Answer 10

high in restriction sites where restriction enzymes cut. genes are inserted

Question 11

where is the gene inserted after the plasmid is cut

Answer 11

into plasmid dna
- ends are complementary to the opened plasmid

Question 12

hoe many hydrogen bonds are between c and g

Answer 12

3

Question 13

how many hydrogen bonds are between a and t

Answer 13

2

Question 14

what kind of bond is between phosphates and sugars

Answer 14

phosphodiester

Question 15

what kind of bond is between bases

Answer 15

hydorgen bonds

Question 16

recombinant dna

Answer 16

combining dna from two different sources into one dna molecule

Question 17

steps to clone dna into a vector/plasmid

Answer 17

1. cut out gene in dna with restriction enzymes
2. cut open the vector, making it linear ————
3. stick vector and gene together with dna ligase