DNA cont. Flashcards

1
Q

introns

A

noncoding regions of dna/rna

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2
Q

exons

A

coding regions translated and expressed

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3
Q

spliceosomes

A

cut out introns, joins exons

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4
Q

what are added to the primary transcript to protect him digestion

A

5’ cap, 3’ poly a tail from poly a polymerase (made of adenine ribonucleotides)

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5
Q

mutagens

A

chemicals/radiation that increase likelihood of a mutation occurring

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6
Q

purines pair with pyrimidines to increase ______

A

stability

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7
Q

bacterial plasmids

A

extra chromosomal dna in the cytoplasm of bacterial cells

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8
Q

selection site

A

gene for antibiotic resistance

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9
Q

endosymbiotic relationship

A

both benefit
- eg. bacteria and plasminds

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10
Q

cloning region

A

high in restriction sites where restriction enzymes cut. genes are inserted

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11
Q

where is the gene inserted after the plasmid is cut

A

into plasmid dna
- ends are complementary to the opened plasmid

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12
Q

hoe many hydrogen bonds are between c and g

A

3

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13
Q

how many hydrogen bonds are between a and t

A

2

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14
Q

what kind of bond is between phosphates and sugars

A

phosphodiester

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15
Q

what kind of bond is between bases

A

hydorgen bonds

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16
Q

recombinant dna

A

combining dna from two different sources into one dna molecule

17
Q

steps to clone dna into a vector/plasmid

A
  1. cut out gene in dna with restriction enzymes
  2. cut open the vector, making it linear ————
  3. stick vector and gene together with dna ligase