DNA-based methods: Quantitative fluorescent PCR

Question 1

QF-PCR has been used to detect aneuploidy involving what chromosomes?

Answer 1

21, 18, 13 and sex chromosomes

Question 2

In this method, several polymorphic short tandem repeat microsatellite markers distributed over the chromosomes in question are amplified by PCR using primers labelled with one of a range of fluorescent dyes. This is followed by accurate determination of the size and relative abundance of the DNA products on an automated sequencer

Answer 2

QF-PCR

Question 3

Typically, ____, i.e. a combination of several simultaneous PCR reactions within one tub, for markers on chromosomes 13, 18 and 21 is carried out.

Answer 3

multiplex PCR

Question 4

In QF-PCR, the diagnosis of a trisomy requires a clear ____ on a chromosome indicated by either three peaks (representing three alleles) or two peaks (with one peak being of approximately twice the size of the other), for at least two informative markers

Answer 4

trisomic pattern