Array comparative genomic hybridization Flashcards

1
Q

In this technique, a subject’s test DNA is labelled with a green fluorescent dye and mixed with reference genomic DNA labelled in red. The mixture is then hybridized to a microarray of 3, 000 genomic DNA sequences. Finally, the fluorescence is measured at each spot, with the green:red ratio indicating the relative abundance of subject versus reference DNA.

A

high-resolution aCGH

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1
Q

Submicroscopic deletions and duplications located anywhere in the genome can be detected using ____.

A

high-resolution aCGH

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2
Q

____ is based on sequence and marker data from the Human Genome Project. It utilises microarrays of thousands of DNA sequences, spaced at intervals along the chromosomes, spotted onto slides

A

Array CGH

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3
Q

T/F. Arrays vary in resolution, from 1 Mb between sequences, to tiling path arrays with a resolution of about 1kb.

A

True

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4
Q

In this technique, the ratios from all the spots can be plotted against a chromosome map to reveal regions of copy-number variation. The method identifies pathological duplications and deletions, which may be difficult to distinguish from non-pathological copy-number variation without parental samples or reference to population control data.

A

Array CGH

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4
Q

T/F. Regions of deletion or duplication detected by a CGH can, if appropriate, be confirmed by FISH.

A

True.

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5
Q

T/F. aCGH is increasingly being used to identify pathogenic regions of copy-number alteration in patients with unexplained learning disability and dysmorphic features. It can also be used to detect such alterations at chromo- some breakpoints in patients with apparently balanced reciprocal chromosome translocations.

A

True.

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