DNA And RNA Flashcards
What happened when Griffith injected mice with a mixture of heat killed, pneumonia causing bacteria and live bacteria of the harmless type?
The mice died.
What happen when Griffith injected mice with the pneumonia causing strain of bacteria that had been heat killed?
The mice lived.
What did Griffith call the process where the heat kill bacteria gave the disease to the harmless bacteria.
Transformation
What experiments did Avery do?
He injected different enzymes that destroyed certain molecules into different strains of pneumonia causing bacteria. The only mouse that lived was the one given the DNA destroying enzyme.
What did Avery conclude?
That DNA stores in transmits genetic information from one generation to the next.
What was the purpose of Oswald Avery’s experiments?
He wanted to determine what molecule in the heat killed bacteria was responsible for the transformation.
What is a virus that infects bacteria?
A bacteriophage
What did Hershey and Chase want to find out?
What part of the virus Internet bacteria.
Describe Hershey and Chase’s experiment.
- they grew viruses in cultures containing radioactive isotopes
- this way they could trace for DNA and the protein
- then they let it Infect the bacteria
What did Hershey and Chase conclude?
The genetic material material of the bacteriophage was indeed DNA, not protein.
What does a bacteriophage consist of?
A DNA or RNA core surrounded by a protein coat.
Describe the lytic cycle.
- the bacteriophage attaches to the bacteria and injects its DNA
- The virus gets into the host cell, and all it wants to do is reproduce
- The viral genes then take over the bacteria and cause it to make virus parts
- doesn’t kill the cell immediately
Describe the lysogenic cycle.
- The virus injects its DNA
- The viral DNA fuses with the bacterial DNA
- When the bacteria reproduces it copies the viral DNA
- Eventually the viral DNA triggers the cell to enter the Lytic cycle
- The cell can re-enter the lysogenic cycle
What is DNA made of?
A polymer of subunits called nucleotides
What are the three parts of a nucleotide?
- 5 carbon sugar
- phosphate group
- nitrogenous bases
What is a purine?
A nitrogenous base that contains two carbon rings
What is example of a purine?
Adenine and guanine
What is a pyrimidine?
A nitrogenous base containing one carbon ring
What is an example of a pyrimidine?
Thymine and cytosine
What did Erwin Chargaff discover?
That the percentages of guanine and cytosine were almost equal and any sample of DNA, the same thing applied to thymine and adenine.
Who were Wilkins and Franklin and what did they discover?
They were both scientists who were working independently with DNA diffraction, and they produced an image.
What did Watson and Crick discover?
They built a 3-D model of DNA, and using the work of Franklin and Wilkins they determine that DNA was a double helix and that the backbone of DNA consist of alternating sugar and phosphate, while nitrogenous bases form the “rungs” of the helix.
What does the word bacteriophage mean?
Bacteria eaters
What are the seven parts of a bacteriophage?
- head
- DNA
- neck
- collar
- sheath
- tail fiber
- base plate
What holds DNA together?
Hydrogen bonds
Why is DNA so easy to replicate?
It’s structure is very simplistic.
What is the shape of a eukaryotic chromosome?
X
What is the shape of a prokaryotic chromosome?
O
What is the first step of DNA replication?
The DNA is “unzipped” by the enzyme helicase which breaks the hydrogen bonds in several locations called replication bubbles.
What is the second step of DNA replication?
DNA polymerase attaches to the old strands and “plugs” in the appropriate base pairs.
What is the only way that DNA polymerase can move?
3’ to 5’
True or false
The two DNA strands run in the same direction.
False, the DNA strands move in opposite directions and this is called anti-parallel.
What is the strand that gets copied in fragments called?
The lagging strand.
What are the fragments of DNA getting copied on the lagging strand called?
Okazaki fragments
What links together the Okazaki fragments?
DNA ligase