Diarr Protozoans (lec 7)

Question 1

Giardia lamblia microbe characteristics?

Answer 1

flagellate protoza

Question 2

G. lamblia morphology stages? (2)

Answer 2

1) Trophozoite:
In SI, eats mucus, Ø tissue invasion

2) Cyst:
In environment, makes 2 trophozoite, 4 nuclei when mature

Question 3

G. lamblia Life Cycle? (4 steps)

Answer 3

1) Ingestion (infection)
2) Excystation in stomach
3) Replicate in SI bile salt and neutral pH
4) Cysts -> poop

Question 4

G. lamblia sxs?

Answer 4

aysmp to acute/chronic D

Question 5

G. lamblia action?

Answer 5

causes malabsorption

Question 6

G. lamblia epidemiology?

Answer 6

highly contagious,
contaminated H2O,
(U) kids,
travel, camp, oral

Question 7

G. lamblia diagnostics? (2)

Answer 7

Stool sample

ELISA for GSA65 antigen

Question 8

G. lamblia tx?

Answer 8

DOC: metronidazole

Human milk has IgA against,
Develop post-exposure Ab

Question 9

Amebiasis/

E. histolytica morph stages?

Answer 9

1) Trophozoite:
invasive/pathogenic/motile
pseudopod-forming

2) Cyst:
infectious stage

Question 10

E. histolytica sxs?

Answer 10

Widespread (intestinal/extraintest)
asymp to colitis, abscess, ulcers

(U) bloody D

Question 11

E. histolytica epidemiology?

Answer 11

Fecal H2O
reportable
kids, compromised

Question 12

E. histolytica Life Cycle?

Answer 12

1) Ingest cysts
2) Excystation in Colon
3) Trophoz colonize colon mucus
4) Encyst -> poop or disseminate to blood

Question 13

E. histolytica diagnostics?

Answer 13

stool sample
ELISA
radiology for extraintest

Question 14

E. histolytica tx?

Answer 14

DOC: metronidazole + iodoquinol

Must boil H2O -> resistant to Cl-

Question 15

E. histolytica virulence factors? (4)

Answer 15

1) Lectin: adhesion
2) Phospholipases: disrupt host membrane
3) Amebapore: makes channels in host (lysis)
4) Cysteine Proteases: degrade mucus, Ab, complement, cell matrix

Question 16

Cryptospororidiosis/
C. homins
C. parvum
morphology?

Answer 16

1) Oocysts (infectious, only need 10):
ea makes 4 sporoz

2) Sporoziotes:
bind intestine epi cells

Question 17

C. homins/C. parvum sxs?

Answer 17

Watery D w/ severe wasting
5-10 days
shed for wks

Question 18

C. homins/C. parvum epidemiology?

Answer 18

C. homins - human host
C. parvum - human and bovine hosts

Endemic: P-to-P (fecal-oral)
Sporadic: cow H2O, pools (Cl- resist)

(U) kids, AIDS/compromised

Question 19

C. homins/C. parvum diagnostics?

Answer 19

Oo in stool
Immunofluo
ELISA

Question 20

C. homins/C. parvum Life Cycle?

Answer 20

1) Ingest Oo
2) Releases 4 sporo
3) Bind epi cells
4) Ingested into vacuole (separate from cytoplasm)
5) Sex rep -> thick-walled Oo -> shed
Asex -> thin-walled Oo -> reinfect host
NO MULTIPL OUTSIDE HOST

Question 21

C. homins/C. parvum tx?

Answer 21

DOC: Nitazoxanide or Paroromycin

poor prog is compromised

Question 22

Cylcosporiasis/

C. cayetanensis morph?

Answer 22

large, autofluo Oocysts

Question 23

C. cayetanensis Life Cycle?

Answer 23

Ingest Oo
same progression

Oo MUST sporulate in environment

Question 24

C. cayetanensis sxs?

Answer 24

Explosive, watery D + viral sxs

Question 25

C. cayetanensis epidemiology?

Answer 25

(U) latin america

Contaminated H2O, fruit/veg

Question 26

C. cayetanensis diagnostics?

Answer 26

Large Oo in stool

Acid-fast fluor Oo

Question 27

C. cayetanensis tx?

Answer 27

DOC: Trimeth-Sulfameth

Question 28

Blastocystis hominis morph?

Answer 28

Large central vacuole,

multiple nuclei around rim

Question 29

B. hominis epidemiology?

Answer 29

animal fecal H2O

Question 30

B. hominis tx?

Answer 30

Metronidazole

Question 31

Balantidium coli morph?

Answer 31

Large Trophozoites w/ cilia and MACROnucleus

and Cysts

Question 32

B. coli epidemiology?

Answer 32

Pig poop

Question 33

B. coli tx?

Answer 33

TCN or Metronidazole