Diagnostics: Serology and Immunohistochemical Flashcards

1
Q

Pathognomonic signs

A

Diagnostic sign marking the presence of a particular disease

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2
Q

Silver dollar plaques on a horse indicate…

A

Dourine: Chronic veneral disease

Trypanosomoma equiperdum

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3
Q

Pink diamond-shaped skin lesions in pigs indicate…

A

Swine erysipelas: diamond skin disease

Erysipelothrix rhusiopathiae

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4
Q

Types of specimens for collection

A
Tissues
Blood
Scrapes/Swabs/Impression
Transudate/Exudates
Urine/misc fluids
Feces
Vomit/sputum
Blood
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5
Q

Factors that influence specimen collection choice

A

Clinical symptoms
Duration of infection
Diagnostic tests to be performed

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6
Q

What is the first step in accurate diagnostic testing?

A

Proper specimen collection!

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7
Q

Specimens for BACTERIAL infections

A

Dog/Cat: skin scrape, hair, ear swab, urine, blood, wound swabs

Horses: nasal swabs and fluids, wound swabs

Food animals: Post mortem tissues/organs, milk

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8
Q

Specimens for PARASITIC infections

*base on parasitic life cycle

A
Feces
Vomit
Sputum
Blood
Urine
Skin scrape
Muscle biopsy
Post mortem samples
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9
Q

Specimens for VIRAL infections

A
Feces
Blood
Nasal, tracheal, eye swabs
Sputum
Post mortem samples
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10
Q

At what phase of disease is the best time to collect samples?

A

During the acute phase

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11
Q

Goals of aseptic sampling

A

Reduce/avoid contamination with normal flora

Avoid environmental contamination

Reduce risk of secondary infection

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12
Q

Correct handling and transport depends on…

A

Pathogen type
Specimen type
Diagnostic tests being performed

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13
Q

3 types of diagnostic tests

A

Phenotypic
Molecular
Serology/immunohistochemical

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14
Q

What do immunohistochemical tests detect?

A

Pathogen-specific antibodies or antigens

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15
Q

Why choose immunohistochemical methods?

A

Quickly diagnose threatening agents

Organisms take too long to culture (mycobac)

Organisms are difficult to culture (Rickettsiae)

Unculturable organisms (Clostridium piliformis)

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16
Q

The ability of the test to detect very minute quantities of antigen or antibody

A

Sensitivity

17
Q

The ability of the test to detect reactions between homologous Ag and Ab, minimizing false positives

A

Specificity

18
Q

What specimens detect antibodies?

A

Blood, tissue fluids

serology

19
Q

What specimens detect antigens?

A

Areas of infection where pathogens replicate and are present

serotyping

20
Q

Best time to collect to detect antigen

A

Acute phase

21
Q

Best time to collect to detect antibody

A

10-14 days after infection

22
Q

Indicators for active or recent infection

A

Pathogen detection

Clinical symptoms

Antibody titer
*number of circulating antibodies will decrease over time. Presence may not indicate active infection. Absence may not mean absence of pathogen

23
Q

What test detects Ab response to viruses, parasites, bacteria or fungi?

A

ELISA (Enzyme Linked ImmunoSorbent Assay)

24
Q

Benefits of ELISA

A

High sensitivity and specificity

Quantitative: indicates amount of Ag or Ab present

25
Q

Types of ELISA

A
Direct
Indirect
Sandwich
Lateral-flow
IDEXX snap test
26
Q

In which type of ELISA is the antigen bound to the solid phase, washed, then directly incubated with conjugated antibody?

A

Direct

27
Q

Features of DIRECT ELISA

A

qualitative or quantitative Ag detection

antibody screening

epitope mapping

28
Q

What makes INDIRECT ELISA more sensitive than direct ELISA?

A

Addition of labeled second antibody enhances the signal of the primary antibody

29
Q

What type of ELISA was designed for soluble antigens or low concentrations, and requires a compatible antibody pair that recognizes different epitopes on the same antigen?

A

Sandwich

30
Q

Variation of ELISA that is mostly qualitative or semi-quantitative and confirms presence or absence of target molecule

A

Lateral-Flow Immunoassay

31
Q

Benefits of lateral flow immunoassay

A
User friendly
One-step analysis
Low cost
Versatile
Rapid
32
Q

What is a variation of the lateral flow tests that uses bidirectional flow and integrates a wash step to minimize false positives?

A

IDEXX snap tests

33
Q

Benefits of IDEXX snap tests

A
Integrated wash step
Increased sensitivity
Small amount of antigen will work
Variety of tests
Some tests detect multiple diseases
34
Q

Benefits of Immunofluorescence

A

Localize pathogen

Detect autoimmune, viral, diseases (HW, FIP)

High sensitivity and specificity

35
Q

What test employs a visible clumping of particulate Ag with its specific Ab, forming a visible lattice?

A

Agglutination test

36
Q

Benefits of agglutination

A

Easy to make
Easy to use
Cheap
Clearly visible reaction

37
Q

Disadvantages of agglutination

A

Low sensitivity
Low specificity
Affected by vaccine-induced Ab

38
Q

Benefits of immunochemical methods

A
Good for non-culturables
Most have high sensitivity
Most have high specificity
Mid to high volume testing possible
Indication of immune response
39
Q

Disadvantages of immunochemical methods

A

Detection of Ab not always indicative of active infection

Detection limit: Ab might not be detected in samples taken early in infection

Ab can reflect multiple pathogens that have the same antigen