Diagnostics: Serology and Immunohistochemical Flashcards
Pathognomonic signs
Diagnostic sign marking the presence of a particular disease
Silver dollar plaques on a horse indicate…
Dourine: Chronic veneral disease
Trypanosomoma equiperdum
Pink diamond-shaped skin lesions in pigs indicate…
Swine erysipelas: diamond skin disease
Erysipelothrix rhusiopathiae
Types of specimens for collection
Tissues Blood Scrapes/Swabs/Impression Transudate/Exudates Urine/misc fluids Feces Vomit/sputum Blood
Factors that influence specimen collection choice
Clinical symptoms
Duration of infection
Diagnostic tests to be performed
What is the first step in accurate diagnostic testing?
Proper specimen collection!
Specimens for BACTERIAL infections
Dog/Cat: skin scrape, hair, ear swab, urine, blood, wound swabs
Horses: nasal swabs and fluids, wound swabs
Food animals: Post mortem tissues/organs, milk
Specimens for PARASITIC infections
*base on parasitic life cycle
Feces Vomit Sputum Blood Urine Skin scrape Muscle biopsy Post mortem samples
Specimens for VIRAL infections
Feces Blood Nasal, tracheal, eye swabs Sputum Post mortem samples
At what phase of disease is the best time to collect samples?
During the acute phase
Goals of aseptic sampling
Reduce/avoid contamination with normal flora
Avoid environmental contamination
Reduce risk of secondary infection
Correct handling and transport depends on…
Pathogen type
Specimen type
Diagnostic tests being performed
3 types of diagnostic tests
Phenotypic
Molecular
Serology/immunohistochemical
What do immunohistochemical tests detect?
Pathogen-specific antibodies or antigens
Why choose immunohistochemical methods?
Quickly diagnose threatening agents
Organisms take too long to culture (mycobac)
Organisms are difficult to culture (Rickettsiae)
Unculturable organisms (Clostridium piliformis)