Detecting Proteins Using Antibodies

Question 1

What are the ways to detect proteins using antibiotics

Answer 1

Immunoassay, ELISA techniques, western blotting

Question 2

What are immunoassay techniques used to do

Answer 2

To detect and identify specific proteins

Question 3

What do immunoassay techniques use

Answer 3

Stocks of antibodies with the same specificity known as monoclonal antibodies

Question 4

What is an antibody specific to the protein antigen linked to

Answer 4

The chemical label

Question 5

What is the chemical label often

Answer 5

Often a reporter enzyme producing a colour change but chemiluminescence and fluorescence can be used

Question 6

Why does an assay uses specific antigens

Answer 6

To detect the presence of antibodies to test for diseases

Question 7

What’s ELISA

Answer 7

analytical technique which uses antibodies to detect the presence of an antibody within a solution

Question 8

What are the 3 forms of ELISA

Answer 8

direct, indirect and sandwhich

Question 9

What is western blotting

Answer 9

Technique which is used after SDS-PAGE electrophoresis. Once the proteins have been separated in the gel they are transferred or blotted onto a solid medium

Question 10

How can proteins be identified in western blotting

Answer 10

Using specific antibodies with reporter enzymes attached (ELISA)