Aseptic Technique And Cell Culture Flashcards
What is aseptic technique definition
Procedures used in laboratories to reduce contamination as well as the unwanted growth or spread of microorganisms
What do aseptic techniques eliminate
Unwanted microbial contaminants when culturing microorganisms or cells
What does aseptic technique involve
The sterilisation of equipment and culture media by heat or chemical means and subsequent exclusion of microbial contaminants
What is microbial culture definition
A method of multiplying microorganisms by letting them reproduce in a culture medium under controlled laboratory conditions
How can a microbial culture be started
Using an inoculum of microbial cells on an agar medium or in a broth with suitable nutrients.
What do many cultures exist to do
Promote growth of specific types of cells and microbes
Where are animal cells grown
In medium containing proteins called growth factors from serum
What are proteins that grow animal cells promote and are essential for what
Promote cell growth and proliferation. They are essential for the culture of most animal cells
In culture what can primary and tumour cell lines to
Primary can divide a limited number of times
Tumour can perform unlimited divisions
What does plating out of liquid microbial culture on a solid medianallow
The number of colony forming units to be counted and the density of cells in the culture estimated
What is a serial dilution needed for
To achieve a suitable colony count
What’s haemocytometers
Are specialised microscope cells which are used to estimate cell numbers in a liquid culture
How do you use haemocytometers
-Calculate the volume of each of the large squares in the grid by multiplying are by depth (0.1mm)
-Count the number of cells,s I’m each corner grid including those that are touching the top and left sides and calculate the average
-calculate cell density = average cells per square/ volume of the small square
What is required in order to identify and count living cells
Vital straining
What do living and non living cells do to strains
Living cells will absorb the strain whilst non living cells do not
