Dental Plaque and Biofilms (3) Flashcards

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1
Q

What type of plaque is hard to investigate?

A

subgingival plaque

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2
Q

What makes the subgingival environment different to the supragingival environment?

A

Protected from friction
Attachment and adherence is less important
Different nutrients available
Eh - Lower oxygen concentration (different redox potential
pH - alkaline
Different host defence mechanisms

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3
Q

What are the initial colonisers of subgingival plaque?

A

Gram –ve rods and cocci and Spirochaetes are the initial colonisers

more motile bacteria

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4
Q

What type of anaerobe does the more complex subgingival plaque consist of?

A

With time subgingival plaque becomes more complex and consists predominantly of obligatory or facultative anaerobes

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5
Q

When does subgingival plaque become established?

A

Subgingival plaque may become established as early as 4 weeks after the onset of gingivitis

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6
Q

How many bacteria are motile in the subgingival plaque?

A

50%

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7
Q

Why does the subgingival plaque have no or little matrix production?

A

it doesn’t have the frictional forces on it

therefore the bacteria are loosely adherent to the tooth surface

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8
Q

What fluid delivers the microbes to the subgingival area?

A

the GCF

gingival crevicular fluid

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9
Q

What does the GCF deliver?

A

Humoral and cellular defence factors

Proteins and glycoproteins that serve as novel substrates for microorganisms e.g. haem containing molecules

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10
Q

What cellular defence factors does the GCF provide?

A

neutrophils

WBC

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11
Q

What do glycoproteins act as to bacteria in the subgingival area?

A

act as food

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12
Q

When do haem containing molecules arise?

A

in inflammatory areas

lots of blood

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13
Q

What type of bacteria are associated with periodontal diseases?

A

asacchrolytic

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14
Q

What do asacchrolytic microbes feed on?

A

not sugars but proteins

proteolytic

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15
Q

How does proteolysis effect pH?

A

more alkaline environment

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16
Q

What bacteria does the more alkaline conditions favour in the subgingival pocket?

A

Porphyromonas gingivalis

(periodontal pathogens)

17
Q

Does the temperature increase or decrease during inflammation?

A

increase

due to proteolytic metabolism

18
Q

Compare the supra- and subgingival plaque with regards to their matrix

A

supra = 50% matrix
sub = little or no matrix

19
Q

Compare the supra- and subgingival plaque with regards to their flora

A

supra = mostly Gram +ve rods and cocci
sub = mostly Gram -ve and spirochaetes

20
Q

Compare the supra- and subgingival plaque with regards to their motile bacteria

A

supra = few
sub = common

21
Q

Compare the supra- and subgingival plaque with regards to their aerobic/anaerobic bacteria

A

supra = aerobic unless thick
sub = anaerobic

22
Q

Compare the supra- and subgingival plaque with regards to their metabolism

A

supra = mostly carbohydrate
sub = mostly protein

23
Q

Compare the supra- and subgingival plaque with regards to their species diversity

A

supra = little but increasing with age
sub= great