Day 3

Question 1

Myoglobin vs Hemoglobin (Oxygen affinity)

Answer 1

Myoglobin is higher, it is in the muscles

Question 2

acetylcholine

Answer 2

muscular contractions, neurotransmitter

Question 3

acetylcholineesterase

Answer 3

breaks down acetylcholine

Question 4

How to compete with competitive inhibitors?

Answer 4

increase substrate

Question 5

Competitive Enzymes? KM and Vmax?

Answer 5

Vmax stays the same(still have same enzyme conc.) Km Goes up (less affinity, since more competition)

Question 6

Everything but competitive inhibitors w/ Vmax?

Answer 6

Vmax decreases

Question 7

Non-Competitive Inhibitor graph?

Answer 7

Question 8

Mixed Inhibitor Graph?

Answer 8

Intersection is weird, not at km or Vmax point, not parallel. Can increase or decrease km

Question 9

Uncompetitive Inhibitor

Answer 9

Question 10

Thin Layer Chromatography (TLC)

Answer 10

One Close to original dot is better aligned with the mobile phase. The one that has travelled the furthest is more aligned with the stationary phase. Rf is distance traveled/max distance

Question 11

Column Chromatography

Answer 11

Seperation for purification

Question 12

Gas Chromatography is usually paired with?

Answer 12

Mass Spectrometer. Gas seperates it MS IDs it.

Question 13

High Performance Liquid Chromatography (HPLC)

Answer 13

For very small samples, separates

Question 14

-Ane & -Ene ; polar or non-polar?

Answer 14

non-polar

Question 15

Distillation (requirements?)

Answer 15

25 degrees apart, under 150

(Otherwise, Fractional distillation)

Question 16

Fractional distillation

Answer 16

Question 17

Polyacrylamide Gel Electrophoresis (PAGE)

v.

SDS PAGE

Answer 17

PAGE- seperated by mass/charge (protein can be salavaged) (simple boil) (denatured)

SDS Page- seperated by mass only (destroys protein) (high T and chemicals) (degraded)

Question 18

SNOW DROP

Answer 18

Southern-DNA

Northern-RNA

Western-Protein