Day 3 Flashcards
Myoglobin vs Hemoglobin (Oxygen affinity)
Myoglobin is higher, it is in the muscles
acetylcholine
muscular contractions, neurotransmitter
acetylcholineesterase
breaks down acetylcholine
How to compete with competitive inhibitors?
increase substrate
Competitive Enzymes? KM and Vmax?
Vmax stays the same(still have same enzyme conc.) Km Goes up (less affinity, since more competition)

Everything but competitive inhibitors w/ Vmax?
Vmax decreases
Non-Competitive Inhibitor graph?

Mixed Inhibitor Graph?
Intersection is weird, not at km or Vmax point, not parallel. Can increase or decrease km

Uncompetitive Inhibitor

Thin Layer Chromatography (TLC)
One Close to original dot is better aligned with the mobile phase. The one that has travelled the furthest is more aligned with the stationary phase. Rf is distance traveled/max distance

Column Chromatography
Seperation for purification
Gas Chromatography is usually paired with?
Mass Spectrometer. Gas seperates it MS IDs it.
High Performance Liquid Chromatography (HPLC)
For very small samples, separates
-Ane & -Ene ; polar or non-polar?
non-polar
Distillation (requirements?)
25 degrees apart, under 150
(Otherwise, Fractional distillation)
Fractional distillation

Polyacrylamide Gel Electrophoresis (PAGE)
v.
SDS PAGE
PAGE- seperated by mass/charge (protein can be salavaged) (simple boil) (denatured)
SDS Page- seperated by mass only (destroys protein) (high T and chemicals) (degraded)
SNOW DROP
Southern-DNA
Northern-RNA
Western-Protein