Day 2 Flashcards
Most Common Quartnery Protein?
Hemoglobin
Coenzymes Vs Cofactors
Coenzymes are organic (have carbons, such as vitaimes)
Cofactors are inorganic (no carbon, like metals)
Fat Soluble Vitamins?
DEKA
Disadvantage of too much fat soluble vitamins?
Fats are not excreted, get stored in fat cells
Coenzymes and Cofactors, Their Role
Enzyme cannot bind to substrate without coE or coF present
holoenzyme
is whole, has its cofactor or coenzyme
apoenzyme
empty, does not have necessary cofactor or coenzyme
prosthetic group
when enzymes basically permanently bound, i.e iron and hemoglobin
lil hot
ligase, isomerase, lyase, hydrolase
Ligase
joins things together (i.e DNA ligase, synthetase)
Isomerase
Rearrange, change structure, shufflers (mutase)
Lyase
Breaks things apart without water
hydrolase
breaks things apart with water (amylase,peptidease)
Oxidoreductase
promotes redox reactions (dehydrongenase)
Transferase
Transfers a functional group (kinase-add phosphate groups)
phosphorolase
breaks bond by adding phosphate
pepsin
in your stomach, pH 2
Body temp C?
37 C
taq polymerase
Operates at HIGH temps (Polymerase Chain Reaction)
Polymerase Chain Reaction (PCR)
Makes lots of copies of DNA at high temps (taq polymerase)
Hill > 1
Cooperative Binding (i.e. hemoglobin)
Lineweaver Burk (X, Y, Y Intercept, X Intercept?)
X= 1/S
Y= 1/V
Y Intercept= 1/Vmax
X Intercept= -1/Km