Day 1: things i forget Flashcards

1
Q

At what temperature do you incubate bile aesculin agar?

A

45 degrees celsius

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2
Q

What agar do you always stab?

A

Urease

Tinsdale (stab primary inoculum)

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3
Q

What agar do you use for optochin and bacitracin susceptibility testong?

A

Blood

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4
Q

What agar do you use for TAXO sugars

A

Chocolate

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5
Q

What do you add to TAXO sugars after incubation?

A

Phenol red

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6
Q

Where do you put your metronidazole discs

A

Only need to put on the test in the middle of the secondary inoculum streaks

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7
Q

What are your controls for anaerobic growth test

A

Bacteroides fragilis = anaerobic growth only
Pseudomonas aeuriginosa = aerobic growth only

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8
Q

How do you inoculate the lactose gelatin

A

Use straight wire loop to stab gel once

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9
Q

What are your controls for the lactose gelatin

A

Lactose positive and gelatinase positive = yellow liquid = C. perfringens

Lactose negative and gelatinase positive = red liquid = C. sporogenase

Lactose positive and gelatinase negative = yellow gel = E. Coli

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10
Q

What do you need to remember about trehalose and urease tests?

A

You need a blood agar purity plate

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11
Q

What agar did we use for growth at different temperatures for pseudomonas?

A

Nutrient agar

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12
Q

How do you inoculate your nutrient agar for growth at different temperatures for pseudomonas

A

Zig-zag inoculum

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13
Q

What is your diptheroid negative control for Tinsdale agar?

A

Staph aureus

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14
Q

How do you inoculate citrate slope for enterobacterales?

A

Zig zag and stab
lave lid loose

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15
Q

How do you carry out the biochemical tests for enterobacterales?

A

Ask for straight loop, oil, MacConkey agar plate and chemicals

Inoculate a sterile water

  • Peptone (water) indole
  • Buffered glucose Methyl Red
  • Buffered glucose Voges Proskauer Test
  • Peptone sugar dulcitol
  • Lysine decarb base control (oil layer)
  • Lysine decarb test (oil layer)
  • Phenylalanine agar
  • Urea agar (stab)
  • MacConkey purity plate
  • Citrate slope (zig-zag and stab)
  • Kovacs reagent 8 drops on indole
  • MR reagent on Methyl red
  • VP1 and VP2 reagent on VPT
  • Ferric chloride 3-4 drops for phenylalanine
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16
Q

What agar do you need for the X and V factor test?

A

DST agar
Diagnostic sensitivity agar

17
Q

What agar do you use for your MID8 Mastring test

A

Blood agar

18
Q

What six antimicrobials make up the MID8 ID Mastring test

A

Erythomycin

Eifampicin

Colistin sulphate

Penicillin

Kanamycin

Vancomycin

19
Q

What five antimicrobial disks are used to test for resistance in staphylococci

A

Cefoxitin 30ug

Ciproflaxacin 5 ug

Erythromycin 15ug

Gentamicin 10ug

Penicillin 1 ug

20
Q

What five antimicrobial disks are used to test for resistance in enterbacterales

A

Ampicillin ug
Cefotaxime ug
Ceftazidine 10ug
Gentamicin 10ug
Ertapenem 10 ug

21
Q

What agar do you use for disk diffusion?

A

Mueller-Hinton agar

22
Q

What standard do you use for disc diffusion

A

0.5 McFarland Standard

23
Q

What is the name of the antimicrobial strip used for MIC testing?

24
Q

What bacteria cause otitis media

A

Predominant bacteria that cause otitis media are Streptococcus pneumoniae, Moraxella catarrhalis, and non‐typeable Haemophilus influenzae.

25
How can you identify Haemophilus
Very small colonies Grey
26
How do you identify moraxella
Big white push able colonies