Day 1: things i forget Flashcards
At what temperature do you incubate bile aesculin agar?
45 degrees celsius
What agar do you always stab?
Urease
Tinsdale (stab primary inoculum)
What agar do you use for optochin and bacitracin susceptibility testong?
Blood
What agar do you use for TAXO sugars
Chocolate
What do you add to TAXO sugars after incubation?
Phenol red
Where do you put your metronidazole discs
Only need to put on the test in the middle of the secondary inoculum streaks
What are your controls for anaerobic growth test
Bacteroides fragilis = anaerobic growth only
Pseudomonas aeuriginosa = aerobic growth only
How do you inoculate the lactose gelatin
Use straight wire loop to stab gel once
What are your controls for the lactose gelatin
Lactose positive and gelatinase positive = yellow liquid = C. perfringens
Lactose negative and gelatinase positive = red liquid = C. sporogenase
Lactose positive and gelatinase negative = yellow gel = E. Coli
What do you need to remember about trehalose and urease tests?
You need a blood agar purity plate
What agar did we use for growth at different temperatures for pseudomonas?
Nutrient agar
How do you inoculate your nutrient agar for growth at different temperatures for pseudomonas
Zig-zag inoculum
What is your diptheroid negative control for Tinsdale agar?
Staph aureus
How do you inoculate citrate slope for enterobacterales?
Zig zag and stab
lave lid loose
How do you carry out the biochemical tests for enterobacterales?
Ask for straight loop, oil, MacConkey agar plate and chemicals
Inoculate a sterile water
- Peptone (water) indole
- Buffered glucose Methyl Red
- Buffered glucose Voges Proskauer Test
- Peptone sugar dulcitol
- Lysine decarb base control (oil layer)
- Lysine decarb test (oil layer)
- Phenylalanine agar
- Urea agar (stab)
- MacConkey purity plate
- Citrate slope (zig-zag and stab)
- Kovacs reagent 8 drops on indole
- MR reagent on Methyl red
- VP1 and VP2 reagent on VPT
- Ferric chloride 3-4 drops for phenylalanine
