Cytoskeleton Flashcards
Which subunit of microtubules is usually bound to GDP?
Beta
What stabilizes the + end of microtubules?
GTP Cap
What are the 3 microtubule cutters?
Katanin
Spastin
Fidgetin
What binds to the - end of microtubules?
Gamma tubulin
What is an example of the cytoskeletal component of microtubules?
Axon outgrowth
True or false: Kinesins are monomers and use ATP
False: they have 2 feet and need to walk. They DO use ATP though.
What is NGF and why is it important for nerves?
Nerve Growth Factor- if an axon doesn’t reach its target (something that secretes NGF), then it will die.
What do astral microtubules bind to?
plasma membrane
What are the 2 types of microtubules that are most important in cell division?
Kinetochore and overlap microtubules.
True or False: intermediate filaments have NO polarity
True
Bob died from testicular cancer that metastasized to his heart and then cause big problems there. How did researchers know that the tumor in his heart was testicular cancer and not heart cancer?
They looked at the keratin expression of the tumor and saw that it matched the testicles.
What are the steps of actin filament formation?
- Nucleation: Take a g-actin, and adding two other monomers. Result is trimer.
- Extension/retraction
What kind of actin does ARP2/3 make?
Branched. ARP binds to 1 actin monomer
What kind of actin does FH2 or formin make?
elongated actin. Formin binds to 2 actin monomers.
What activates Formin?
Small monomeric GTPases
active with GTP
