Cytology,homogenisation And Fractionation

Question 1

Organelles

Answer 1

Membrane bound Living structures of a cell which are situated within the cytoplasm

Question 2

Aims of homogenisation

Answer 2

-highest degree of cell breakage
-minimum disruptive forces
-no damage to organelles

Question 3

Choice of method

Answer 3

-number of samples to be homogenised
-how difficult the cells are to be homegenised
-impact of the method
-how stable the organelle/product is
-how dangerous are the cell/products and methods used
-cost

Question 4

Cell disruption methods

Answer 4

Mechanical
>solid shear eg bread mill
>liquid shear eg ultrasoinification
Non mechanical
>physical eg thermolysis,decompression,osmotic shock
>chemical eg antibodies, detergents
>enzymatic eg lyric enzymes