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Mycology & Virology > Culture Media > Flashcards

Culture Media Flashcards

1
Q

Used for the cultivation of ascosporogenous yeasts such as Saccharomyces cerevisiae.

A

Acetate ascopore agar

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2
Q

better sporulation medium than sodium acetate.

A

potassium acetate

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3
Q

Ascopores from acetate ascopore agar are stained with

A

Kinyoun carbol fuschin acid fast stain

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4
Q

Selective and differential medium used for the isolation of Cryptococcus spp

A

Birdseed, Niger seed agar

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5
Q

C. neoformans and C. gattii are is unique in that they produce the enzyme

A

phenol oxidase

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6
Q

The breakdown of substrate (Guizotia abyssinica seeds or niger seeds) produces this, imparting a tan to brown pigmentation to the colonies

A

melanin

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7
Q

selective ageint in birdseed agar that inhibits bacterian and other fungi

A

chloramphenicol

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8
Q

enhances melanization of C. neoformans in birdseed agar

A

creatinine

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9
Q

Selective and differential medium used for the isolation and differentiation of Candida spp.

A

Bismuth sulfite-glucose-glycine yeast (BiGGY) agar

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10
Q

nutritive base of BiGGY

A

peptone
glucose
yeast extract

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11
Q

Candida reduce ____ to _____

A

bismuth sulfite, bismuth sulfide

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12
Q

inhibitor of bacterial growth in BiGGY

A

bismuth sulfite

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13
Q

appearance of C. albicans in BiGGY

A

black, no pigment diffusion, no sheen

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14
Q

appearance of C. tropicalis in BiGGY

A

dark brown colonies with black center
black pigment diffusion
sheen

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15
Q

used for the cultivation and isolation of all fungi including fastidious dimorphic fungi.

A

Brain Heart Infusion Agar with 10% sheep red cell

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16
Q

inhibitors present in BHI

A

chloramphenicol and gentamicin (bacteria)
cycloheximide (saprobes)

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17
Q

Adaptation of birdseed agar.

A

caffeic agar

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18
Q

the biologically active chemical in niger seed that causes the yeast colony of C. neoformans to turn brown

A

caffeic

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19
Q

Differential medium to distinguish C. neoformans from C. gattii.

A

Canavanine-glycine-bromthymol blue agar

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20
Q

incubation time and temperature of Cryptococcus on Canavanine-glycine-bromthymol blue agar

A

30oC for 1 to 5 days.

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21
Q

turns Canavanine-glycine-bromthymol blue agar cobalt blue

A

C. gattii (serotypes B and C)

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22
Q

leave the Canavanine-glycine-bromthymol blue agar medium greenish yellow.

A

C. neoformans var. grubii (serotype A) and C. neoformans var. neoformans (serotype D)

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23
Q

Chromogenic, differential and selective medium for the isolation of clinically important yeasts.

A

CHROMagar (BD BBL; BD Diagnostics Systems)

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24
Q

Nutritive base of CHROMagar

A

peptone and glucose

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25
Q

inhibits bacterial growth in CHROMagar

A

Chloramphenicol

26
Q

CHROMagar with fluconazole is available for this fluconazole-resistant fungi

A

C. krusei

27
Q

More sensitive than SDA and helpful in identifying mixed cultures of yeasts

A

CHROMagar

28
Q

CHROMagar enhances the rapid assimilation of _____ by C. glabrata

A

trehalose

29
Q

colonies on CHROMagar should be evaluated at

A

48 h

30
Q

used for Presumptive identification of Cryptococcus, Trichosporon, Rhodotorula spp.

A

Christensen’s urea agar

31
Q

Christensen’s urea agar contains

A

urea and phenol red serving as indicator

32
Q

facilitates separation of certain dermatophytes viz., T. mentagrophytes and T. rubrum.

A

Urea hydrolysis

33
Q

Used for the cultivation and differentiation of T. mentagrophytes from T. rubrum on the basis of pigment production.

A

Cornmeal agar with 1% dextrose

34
Q

used for the differentiation of Candida species on the basis of morphological characteristics.

A

Cornmeal agar with Tween 80

35
Q

specifically incorporated in lieu of dextrose for the demonstration of pseudohyphal, chlamydospores, and arthrospores formation.

A

Tween 80 (surfactant)

36
Q

placing a cover slip over the yeast inoculum to create a microaerophilic environment

A

Dalmau method

37
Q

Used for the differentiation of Aspergillus spp.

A

Czapek-Dox agar

38
Q

sole carbon source in Czapek-Dox agar

A

sucrose

39
Q

the sole nitrogen source in Czapek-Dox

A

sodium nitrate

40
Q

Any bacteria or fungi that can use sodium nitrate as a nitrogen source can grow on this medium.

A

Czapek-Dox agar

41
Q

Screening medium for the recovery, selection, and differentiation of dermatophytes from keratinous specimens.

A

Dermatophyte test medium

42
Q

inhibits saprophytic moulds

A

cycloheximide

43
Q

inhibits Gram-negative bacteria.

A

cycloheximide

44
Q

Morphology and microscopic characteristics are easily identified, but pigmentation cannot be discerned because of the presence of phenol red indicator.

A

Dermatophyte test medium (DTM)

45
Q

Dermatophyte test medium (DTM) is color _____ and turns ______ with dermatophyte growth

A

yellow, red

46
Q

Used for the isolation and growth of lipodependent Malassezia spp.

A

Leeming and Notman medium

47
Q

Components of Leeming and Notman medium

A

ox bile
glycerol monostearate
glycerol
Tween 80
cow’s milk (whole fat)

48
Q

may serve as an alternative to SDA

A

Leeming and Notman medium

49
Q

Selective general-purpose medium used for the isolation of fungi from contaminated specimen.

A

Littman oxgall agar

50
Q

selective agents inhibiting bacteria in Litmann oxgall agar

A

Crystal violet and streptomycin

51
Q

restricts the spreading of fungal colonies in Littman oxgall agar

A

oxgall

52
Q

selective medium specifically formulated for the isolation of dermatophytes but also for the isolation of other pathogenic fungi
from specimens contaminated with saprophytic fungi and bacteria.

A

Mycobiotic or Mycosel agar

53
Q

inhibitor in Mycobiotic or Mycosel agar

A

cycloheximide and chloramphenicol

54
Q

Inhibited fungi in Mycobiotic or Mycosel agar

A

Candida and Aspergillus spp.,
mucoraceous fungi
C. neoformans.

55
Q

Used to stimulate conidium production by fungi and stimulates pigment production in some dermatophytes.

A

Potato Dextrose

56
Q

Commonly used with the slide culture technique to view morphological characteristics.

A

Potato Dextrose

57
Q

lowers the pH of potato dextrose medium thereby inhibiting bacterial growth

A

tartaric acid

58
Q

most common culture media in mycology

A

Sabouraud dextrose agar (SDA)

59
Q

SDA consists of

A

pancreatic digest of casein
peptic digest of animal tissue
dextrose at 4% concentration buffered to a pH of 5.6.

60
Q

the selective components of SDA are

A
  1. Chloramphenicol
  2. Cycloheximide
  3. Gentamicin
  4. Ciprofloxacin
  5. Penicillin and(or) streptomycin

Mycology & Virology (10 decks)

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