Cultivation Results Flashcards
1
Q
MPB - Pasteurella
A
Homogenous Turbidity
2
Q
BA - Pasteurella
A
- S form
- Medium size
- Greyish or Transparent (dew drops)
- !P. multocida=M form
- Without Haemolysis
M. Haemolytica and B. Trehalosi = Beta Hemolysis
3
Q
CHA - Pasteurella
A
- Medium size
- Greyish
4
Q
McC - Pasteurella
A
- Growth of this Genus is suppressed by Bile salts
M. Haemolytica = Red colonies
5
Q
BA - Haemophilus
A
- Create small colonies in the zone of Hemolysis of St. Aureus (or other spp.)
- Small amount of NAD is released into the media
- Satellite Colonies
6
Q
CHA - Haemophilus
A
- S form
- Greyish
7
Q
MPA - Haemophilus
A
- Bacteria can grow around a paper disx impregnated with growth factor X or V
8
Q
BA - Acinobacillus
A
- Small
- Greyish
- Some spp. with Haemolysis
-A. pleuropneumoniae, A. suis, A. equuli - Satellitism
A. Pleuropneumoniae = Required growth factors
9
Q
CHA - Actinobacillus
A
- Small
- Transparent
10
Q
MPB - Klebsiella
A
- Homogenous turbidity
11
Q
MPA - Klebsiella
A
- M form
- No pigmentation
12
Q
BA - Klebsiella
A
- M form
- No Haemolysis
13
Q
EA - Klebsiella
A
- Red colonies
- Lactose +
14
Q
XLD - Klebsiella
A
- Yellow colonies
- Lactose +
15
Q
CLED - Klebsiella
A
- Yellow colonies
- Lactose +
16
Q
MPB - Proteus
A
- Homogenous turbidity
17
Q
MPA - Proteus
A
- Swarming growth
18
Q
BA - Proteus
A
- Swarming growth = Rouss phenomenon
- Color of Agar changes to Brown
- Strong smell
19
Q
EA - Proteus
A
- Pink colonies
- Lactose -
20
Q
XLD - Proteus
A
- S form
- Red/Yellow colonies
- Xylose fermentation
- Lactose -
- H2S+ (Black spots)
21
Q
CLED - Proteus
A
- S form
- Green-Blue colonies
- Lactose -
22
Q
MPB - Salmonella
A
- Diffuse turbidity
23
Q
MPA - Salmonella
A
- S form
- Medium
- Transparent
24
Q
BA - Salmonella
A
- S form
- Medium
- Greyish
- No Haemolysis
25
EA - Salmonella
* Pink colonies
* Lactose -
26
XLD - Salmonella
* Red colonies
* H2S + (Black centre)
* Lactose -
27
McC - Salmonella
* Pink colonies
* Lactose -
28
CLED - Salmonella
* Blue colonies
* Lactose -
29
MPB - Escherichia Coli
* Homogeneous turbidity
30
MPA - Escherichia Coli
* S form
* Medium
* Greyish colonies
31
BA - Escherichia Coli
* S form
* Medium size
* **Beta-Hemolysis! Only in strains that posess HLY plasmids!!!!**
32
EA - Escherichia Coli
* Red colonies
* **Metallic sheen**
* Lactose +
33
XLD - Escherichia Coli
* Yellow colonies
* Lactose +
34
McC - Escherichia Coli
* Red colonies
* Lactose +
35
Sorbitol McC - Escherichia Coli
*** Isolation of E. Coli O157
* They can't ferment Sorbitol**
36
RAF - Escherichia Coli
* Contains 1% Raffinose
* Expression of adherence antigens
-They are often encoded on the same plasmid as Raffinose metabolism
37
MPB - Pseudomonas Aeruginosa
* Homogeneous turbidity
* Membrane
* Pigmentation
38
MPA - Pseudomonas Aeruginosa
* S, M, R form
* Large colonies
* Greenish-Blue pigment
* Fruity or Jasmine odour
39
BA - Pseudomonas Aeruginosa
* S, M, R form
* Larger
* Greyish colonies
* **Beta-Hemolysis**
-**Only P. Aeruginosa + *B. Pseudomallei***
40
EA - Pseudomonas Aeruginosa
* Pale Pink colonies
* Lactose -
41
XLD - Pseudomonas Aeruginosa
* Red colonies
* Lactose -
* **H2S -**
42
Special cultivation for Pseudomonas spp.
* Pseudomonas P and F agar
* Media tht enhances the production of pigments
43
Special cultivation for Burkholderia Mallei
* BA + Glycerine 1%
* Nutrient Agar + Glycerine 1-3%
44
Special Cultivation for Burkholderia Pseudomallei
* Nutrient Agar + Glycerine 3%