Cultivation Results Flashcards

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1
Q

MPB - Pasteurella

A

Homogenous Turbidity

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2
Q

BA - Pasteurella

A
  • S form
  • Medium size
  • Greyish or Transparent (dew drops)
  • !P. multocida=M form
  • Without Haemolysis

M. Haemolytica and B. Trehalosi = Beta Hemolysis

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3
Q

CHA - Pasteurella

A
  • Medium size
  • Greyish
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4
Q

McC - Pasteurella

A
  • Growth of this Genus is suppressed by Bile salts

M. Haemolytica = Red colonies

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5
Q

BA - Haemophilus

A
  • Create small colonies in the zone of Hemolysis of St. Aureus (or other spp.)
  • Small amount of NAD is released into the media
  • Satellite Colonies
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6
Q

CHA - Haemophilus

A
  • S form
  • Greyish
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7
Q

MPA - Haemophilus

A
  • Bacteria can grow around a paper disx impregnated with growth factor X or V
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8
Q

BA - Acinobacillus

A
  • Small
  • Greyish
  • Some spp. with Haemolysis
    -A. pleuropneumoniae, A. suis, A. equuli
  • Satellitism

A. Pleuropneumoniae = Required growth factors

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9
Q

CHA - Actinobacillus

A
  • Small
  • Transparent
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10
Q

MPB - Klebsiella

A
  • Homogenous turbidity
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11
Q

MPA - Klebsiella

A
  • M form
  • No pigmentation
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12
Q

BA - Klebsiella

A
  • M form
  • No Haemolysis
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13
Q

EA - Klebsiella

A
  • Red colonies
  • Lactose +
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14
Q

XLD - Klebsiella

A
  • Yellow colonies
  • Lactose +
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15
Q

CLED - Klebsiella

A
  • Yellow colonies
  • Lactose +
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16
Q

MPB - Proteus

A
  • Homogenous turbidity
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17
Q

MPA - Proteus

A
  • Swarming growth
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18
Q

BA - Proteus

A
  • Swarming growth = Rouss phenomenon
  • Color of Agar changes to Brown
  • Strong smell
19
Q

EA - Proteus

A
  • Pink colonies
  • Lactose -
20
Q

XLD - Proteus

A
  • S form
  • Red/Yellow colonies
  • Xylose fermentation
  • Lactose -
  • H2S+ (Black spots)
21
Q

CLED - Proteus

A
  • S form
  • Green-Blue colonies
  • Lactose -
22
Q

MPB - Salmonella

A
  • Diffuse turbidity
23
Q

MPA - Salmonella

A
  • S form
  • Medium
  • Transparent
24
Q

BA - Salmonella

A
  • S form
  • Medium
  • Greyish
  • No Haemolysis
25
Q

EA - Salmonella

A
  • Pink colonies
  • Lactose -
26
Q

XLD - Salmonella

A
  • Red colonies
  • H2S + (Black centre)
  • Lactose -
27
Q

McC - Salmonella

A
  • Pink colonies
  • Lactose -
28
Q

CLED - Salmonella

A
  • Blue colonies
  • Lactose -
29
Q

MPB - Escherichia Coli

A
  • Homogeneous turbidity
30
Q

MPA - Escherichia Coli

A
  • S form
  • Medium
  • Greyish colonies
31
Q

BA - Escherichia Coli

A
  • S form
  • Medium size
  • Beta-Hemolysis! Only in strains that posess HLY plasmids!!!!
32
Q

EA - Escherichia Coli

A
  • Red colonies
  • Metallic sheen
  • Lactose +
33
Q

XLD - Escherichia Coli

A
  • Yellow colonies
  • Lactose +
34
Q

McC - Escherichia Coli

A
  • Red colonies
  • Lactose +
35
Q

Sorbitol McC - Escherichia Coli

A

* Isolation of E. Coli O157
* They can’t ferment Sorbitol

36
Q

RAF - Escherichia Coli

A
  • Contains 1% Raffinose
  • Expression of adherence antigens
    -They are often encoded on the same plasmid as Raffinose metabolism
37
Q

MPB - Pseudomonas Aeruginosa

A
  • Homogeneous turbidity
  • Membrane
  • Pigmentation
38
Q

MPA - Pseudomonas Aeruginosa

A
  • S, M, R form
  • Large colonies
  • Greenish-Blue pigment
  • Fruity or Jasmine odour
39
Q

BA - Pseudomonas Aeruginosa

A
  • S, M, R form
  • Larger
  • Greyish colonies
  • Beta-Hemolysis
    -Only P. Aeruginosa + B. Pseudomallei
40
Q

EA - Pseudomonas Aeruginosa

A
  • Pale Pink colonies
  • Lactose -
41
Q

XLD - Pseudomonas Aeruginosa

A
  • Red colonies
  • Lactose -
  • H2S -
42
Q

Special cultivation for Pseudomonas spp.

A
  • Pseudomonas P and F agar
  • Media tht enhances the production of pigments
43
Q

Special cultivation for Burkholderia Mallei

A
  • BA + Glycerine 1%
  • Nutrient Agar + Glycerine 1-3%
44
Q

Special Cultivation for Burkholderia Pseudomallei

A
  • Nutrient Agar + Glycerine 3%