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X-Microbiology CT 3 > Biological Tests > Flashcards

Biological Tests Flashcards

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Biology 101 flashcards
1
Q

What biological test is used for Salmonella?

A
  • Sereny’s Test
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2
Q

What is Sereny’s test
Which bacteria is it used for?

A

Sereny’s Test is used for Salmonella Spp., Escherichia Coli

  • Inoculation of broth culture into conjunctival sac of guinea-pigs
  • Positive case = Cataral conjunctivitis to Ulcerose keratoconjunctivitis in 7 days
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3
Q

What biological tests is used for Escherichia Coli?

A
  • Sereny’s test
  • Vero Cell Assay/Detection of Verotoxins
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4
Q

What is Vero Cell Assay
Which bacteria is it used for?

Vero Cell Assay = Detection of Verotoxins

A

Vero Cell Assay is used for Escherichia Coli

  • Verotoxin causes destruction of Verocells
    -A cell line is prepared form Monkey kidney cells
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5
Q

What biological test is used for Burkholderia Mallei and Pseudomallei?

A
  • Strauss reaction
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6
Q

What is Strauss Reaction
Which Bacteria is it used for?

A

Strauss reaction is used for Burkholderia Mallei, Burkholderia Pseudomallei

  • Used for diagnosing Glanders
  • Male guinea-pigs are intraperitoneally infected with either B. Mallei or B. Pseudomallei
  • Positive case = Peritonitis and Orchitis in 4-10 days
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7
Q

What tests are used for Alergenodiagnosis of Glanders?
Burkholderia Mallei

A
  • Mallein test
  • Subcutaneous test
  • Opthalmic test
  • Intrapalpebral test
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8
Q

What is Mallein test
Which Bacteria is it used for?

A

Mallein test is used for Alergenodianosis of Glanders, Burkholderia Mallei

  • Used to demonstrate hypersensitivity developed after infection with B. Mallei
  • Mallein is Glycoprotein extracted from the bacterium
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9
Q

What is Subcutaneous test
Which Bacteria is it used for?

A

Used for Alergenodiagnostic of Glanders, Burkholeria Mallei

  • Subcutaneous application of Mallein
  • Positive case = Swellign + Blisters at inj. site of neck in 24h
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10
Q

What is Ophthalmic test
Which Bacteria is it used for?

A

Used for Alergenodiagnostic of Glanders, Burkholderia Mallei

  • Instillataion of Mallein into the conjunctival sac
  • Positive case = Purulent conjunctivitis in 6-12h
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11
Q

What is Intrapalpebral test
Which Bacteria is it used for?

A

Used for Alergenodiagnostic of Glanders, Burkholderia Mallei

  • Inoculation of Mallein into the skin of lower eyelid
  • Positive case = Oedema, Purulent Keratoconjunctivitis in 48h
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12
Q

Name the method to test for Bacterial resistance agains Antibiotics?

A
  • Kirby-Bauer method
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13
Q

What test is necessary for Pseudomonas Aeruginosa?

A
  • Kirby-Bauer method
    -Testing bacterial resistance against antibiotics
  • Because of multiresistant strains (R factor)
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X-Microbiology CT 3 (19 decks)

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