Cultivation properties

Question 1

Pasteurellaceae

Answer 1

• Form of growth: S, M form
• Relationship to Oxigen: Faculative anaerobes, Microaerophilic (5-10%CO2)
• Range of Temp.: 22-44 C
• Incubation time: 24-48h

Question 2

Klebsiella

Answer 2

• Form of growth: M form
• Relationship to Oxigen: Facultative anaerobes
• Range of Temp.: 10-43 C
• Incubation time: 24h

Question 3

Proteus

Answer 3

• Form of growth: Swarming (S form in XLD+CLED)
• Relationship to Oxigen: Facultative anaerobes
• Range of Temp.: 10-43 C
• Incubation time: 24h

Question 4

Salmonella

Answer 4

• Form of growth: S form
• Relationship to Oxigen: Facultative anaerobes
• Range of Temp.: 20-43 C
• Incubation time: 24h
• Odour: Fecal

Question 5

Escherichia Coli

Answer 5

• Form of growth: S form (M form=capsule)
• Relationship to Oxigen: Facultative anaerobic
• Range of Temp.: 15-45 C
• Incubation time: 24h
• Odour: Fecal
• Capsule: Some strains
• Spores: None

Question 6

Pseudomonas Aeruginosa

Answer 6

• Form of growth: S, M, R form
• Relationship to Oxigen: Strict aerobe
• Range of Temp.: 5-42 C
• Incubation time: 24h
• Odour: Fruity or Jasmine
• Typical features: Pigmentation!
  -Pyocyanin (Blue)
  -Pyoverdin (Yellow)
  -Pyorubin (Red)
  -Pyomelanin (Brown)

Question 7

Pseudomonas Putida
Pseudomonas Fluorescens

Answer 7

• Form of growth: S, M, R form?
• Relationship to Oxigen: Strict aerobe
• Range of Temp.: 4-30 C
• Incubation time: 24h
• Typical feature: Pigmentation
  -Pyoverdin (Fluirescein/Yellow)

Question 8

Burholderia Mallei

Answer 8

• Form of growth: S, M, R form?
• Relationship to Oxigen: Strict aerobe
• Range of Temp.: 22-42 C
• Incubation time: 48h

Question 9

Burkholderia Pseudomallei

Answer 9

• Form of growth: S, M, R form
• Relationship to Oxigen: Strict aerobe
• Range of Temp.: 23-42 C
• Incubation time: 24h
• Odour: Putrid