Cultivation of Bacteria Flashcards
1
Q
Doubling time of E. coli
A
- 20 minutes
2
Q
Doubling time of Mycobacterium tuberculosis
A
- 24 hours
3
Q
What bacteria is present in the decline phase?
A
- viable, but non-cultural bacteria
4
Q
Bacterial nutrition
A
- many pathogens need blood agar to grow
5
Q
Does the environment for bacteria need to be anaerobic or aerobic?
A
- most pathogens are aerobic
6
Q
Microaerophilic coniditions
A
- 5% O2
- 85% Ns
- 10% CO2
7
Q
Thermophilic temperature growth requirements
A
- 45’C
8
Q
Psychrophilic temperature growth requirements
A
- 4’C
9
Q
Short-term preservation
A
- sub-culturing
10
Q
Long-term preservation
A
- freeze-drying (lyophilization)
- 70’C
