Biosecurity

Question 1

Used when agents are exotic and pose serious and/or fatal risk to humans and environment

Answer 1

• BSL 3

- > class II or III BSC

Question 2

Are detergents sporicidal?

Answer 2

• no

Question 3

Used when agents are not known to cause disease in healthy humans

Answer 3

• BSL 1

- > can work outside laminar hood

Question 4

Specially designed facility and all personnel trained with restricted lab access

Answer 4

• BSL 3

Question 5

Last line of defense against pathogen exposure and spread

Answer 5

• Personal Protective Equipment (gloves, lab coat)

Question 6

Methods utilized to protect animals and humans from pathogens and other biohazardous agents

Answer 6

• Biosecurity

Question 7

Survival of pathogenic bacteria in favorable environment (shortest and longest)

Answer 7

1. Mycoplasma - 3 days

2. Bacillus anthracis - over 50 years in soil

Question 8

Does alcohol and phenolics kill spores?

Answer 8

• No, ethyl alcohol, chlorhexidine, hydrogen peroxide, and Virkon all do not kill spores
• > kills bacteria, virus and fungi

Question 9

Used when agents are a moderate risk for healthy humans and environment

Answer 9

• BSL 2

- > class II biosafety cabinet (BSC)

Question 10

A chemical agent for disinfection of living tissue

Answer 10

• antiseptic

Question 11

Kills mycobacterium

Answer 11

• isopropanol

Question 12

Prevents or stops microbial growth

Answer 12

• bacteriostatic

Question 13

A microorganism, virus, toxin, or other biological component that could adversely affect human or animal health

Answer 13

• Biohazard

Question 14

Killing of pathogens on inanimate objects, physical or chemical

Answer 14

• disinfection

Question 15

Used when agents are dangerous, exotic and frequently fatal to humans and have no treatment or vaccine

Answer 15

• BSL 4

- > Class III BSC, double-door autoclave

Question 16

Complete destruction of ALL microbes

Answer 16

• sterilization

Question 17

Kills microbes

Answer 17

• bacteriocidal

Question 18

Restricted access to lab and autoclave present

Answer 18

• BSL 2

Question 19

Prevent contamination of person or object by microbes

Answer 19

• aseptic (asepsis)

Question 20

Specially designed, isolated and sealed facility and highly trained personnel with positive-pressure suits

Answer 20

• BSL 4

Question 21

Used primarily to judge potential risks of biohazardous agents to humans (and environment)

Answer 21

• Biosafety levels

Question 22

Is formaldehyde sporicidal?

Answer 22

• yes

Question 23

70-75% in water is best and is absolute less effective

Answer 23

• ethanol