CRISPR

Question 1

What is CRISPR-Cas9?

Answer 1

Technology that allows editing of genomes and gene modification

Question 2

What is a CRISPR locus made from?

Answer 2

Cas genes, ladder sequence and repeat-spacer array

Question 3

How many CRISPR’s are in a genome?

Answer 3

One or more

Question 4

What are CRISPR’s?

Answer 4

Sequences in the genome of prokaryotic organisms

Question 5

What do CRISPR’s do?

Answer 5

Part of the antiviral prokaryote immune system

Question 6

What is Cas9?

Answer 6

Enzyme that uses CRISPR to recognise and cut complementary DNA to the CRISPR sequences

Question 7

What does PAM stand for?

Answer 7

Protospacer Adjacent Motifs

Question 8

What are PAM’s?

Answer 8

Spacers that prokaryotes select to integrate in their CRISPR locus

Question 9

What are PAM’s made from?

Answer 9

Viral nucleic acids

Question 10

Where are PAM’s found?

Answer 10

Adjacent to short DNA sequences

Question 11

Functions of CRISPR-Cas9

Answer 11

Gene silencing and editing
Transcriptional repression
Activation of endogenous genes
Modification of organisms
Helps with gene-disease