CP2 Microscopy Flashcards
1
Q
What are the 3 skills tested in this practical?
A
- Using compound light microscope safely.
- Measuring actual size of cells.
- Drawing cells.
2
Q
How should you carry the microscope to your bench?
A
- Using both hands.
3
Q
What are the different lenses on a microscope called?
A
- Objective lenses.
4
Q
How do you measure the actual size of cells?
A
- Measure the cell using eyepiece graticule.
- Calibrate graticule scale by alignment of graticule + stage micrometer scales.
5
Q
How do you do a biological drawing of a cell?
A
- Line drawings - no sketchy lines.
- Labels.
- Only draw what you aee.
6
Q
What is the function of a stage micrometer?
A
- Calibrate eyepiece graticule to use for measurements.
7
Q
How to prepare a slide?
A
- Stain sample w/ dye.
- Mount sample on slide.
- Apply coverslip avoiding air bubbles.
8
Q
What is the formula for magnification?
A
- M=I/A
- Magnification = image / actual.
9
Q
What are the requirements for label lines in biological drawings?
A
- Horizontal.
- Straight.
- Not crossing other lines.
10
Q
Outline procedures of focusing w/ light microscope.
A
- Rotate nosepiece to lowest objective lens.
- Look through eyepiece and use coarse focus to focus image.
- Use fine focus knob to fine-tune image.
