core II S3 old Q

Question 1

whats a postive control for a drug that suppresses katanin

Answer 1

with katanin and with katanin and drug and look for breakdown
maybe use gtp analog so they wont breakdown on their one

Question 2

whats a negative control for a drug that suppresses katainin

Answer 2

dish without drug but with vehicle is negative control

Question 3

how can a drug suppress katanin

Answer 3

alter substrate binding domain to prevent katanin hexamer from binding to tubulin (by either directly binding and blocking the binding domain or acting as a negative allosteric modulator)

Question 4

live cell imaging test to tell if a drug prevents microtubules from being severed in cancer cell lines like GFP fusion proteins

Answer 4

express GFP on the tubulin subunits and examine orientation with(out) inhibition in cancer cell line
microtubules should remain visible and not chaing location but inhibtion should dissassemble and appear elsewhere

Question 5

alternative test like a dominant negative approach and why thats good for katanin

Answer 5

dominant negative approach blocks extrinsic and intrinsic katanin behavior
katanin is a good choice because cohexamer subunit is a good target to block normal interactions

Question 6

consequences in skeletal muscle contraction of a mutation that prevents binding of Ca to troponin

Answer 6

blocks SKM contraction

Ca can no longer bind troponin so it stays and blocks tropomyosin which stays and blocks myosin from binding actin

Question 7

name and explain the mechanism of actin polymerization to move stuff

Answer 7

brownian ratchet

flexibility of actin allows bend which allows another subunit to bind and then straitens to exent membrane mechanically

Question 8

extrinsic apoptotic pathway diagram explanation

Answer 8

Fas ligand is pro apoptitic signon aon lymphocytic cell
binds to Fas death receptor
recruits Fas associated death domain FADD
recruitmetn of procaspase 8/10 forming Death receptor induced signal complex DISC
making caspase 8 which activates executioner caspases to apoptosis