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BACTE 1 > Colony Morphology > Flashcards

Colony Morphology Flashcards

1
Q

refers to the inoculation of the clinical specimen onto laboratory media

A

Primary plating

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2
Q

Generally, microbiologists observe the colony
morphology of organisms isolated on primary culture
after how many hours?

A

18 to 24 hours

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3
Q

interpretation of primary cultures

A

Plate reading

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4
Q

laboratory media that contains added agents to inhibit the growth of certain species

A

selective media

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5
Q

permits the differentiation of bacterial strains based on colony morphology based on the ability of some bacteria, and not others

A

differential media

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6
Q

what agar support the growth of fastidious and non-fastidious organisms?

A

SBA and CHOC agar

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7
Q

what is the term called for hard to grown and requires additional growth factors?

A

fastidious organisms

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8
Q

it has the key ingredients of intact sheep red blood cells, vitamin K, and yeast extracted

A

anaerobic blood agar

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9
Q
  • Nutritious medium
  • for the isolation
    and
  • subculturing of
    obligate anaerobic
    bacteria
A

anaerobic blood agar

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10
Q
  • Hemolyzed
    sheep red blood
    cells
  • supplemented
    with Hgb and
    NAD
A

Chocolate agar

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11
Q
  • Primary plating
    and
  • subculturing of
    fastidious
    bacteria,
  • e.g., Haemophilus
    and pathogenic
    Neisseria spp
A

Chocolate agar

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12
Q
  • carbohydrates
    lactose,
  • salicin, and
  • sucrose; and
  • bile salts to
    inhibit growth of
    gram-positive
    bacteria and
    many
    nonpathogenic
    enteric bacteria
A

Hektoen energetic agar

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13
Q
  • selective and
  • differential
    medium
  • for the primary
    plating of stool
    specimens
  • to aid in the
    recovery of
    intestinal
    pathogens,
  • e.g., Salmonella
A

Hektoen enteric agar

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14
Q
  • Lactose and
  • bile salts,
  • the low
    concentration of
    bile salts inhibits
    gram-positive
    bacteria but
  • permits the
    growth of many
    gram-negative
    bacteria
A

MacConkey agar

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15
Q
  • selective and
  • differential
    medium
  • for the isolation
    of gram-negative
    bacteria;
  • used for primary
    plating and
    subculturing
A

MacConkey agar

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16
Q
  • Chocolate agar
    base
  • containing the
    antimicrobial
    agents:
  • vancomycin,
    colistin,
    trimethoprim, and
    nystatin
A

Modified thayer- martin agar

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17
Q
  • selective primary
    plating medium
  • for recovery of
    Neisseria
    gonorrhoeae and
    Neisseria
    meningitidis
A

Modified thayer - martin agar

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18
Q
  • Dextrose
    (glucose),
  • antimicrobials
    can be added to
    inhibit bacteria
A

Sabouraud dextrose agar

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19
Q
  • Primary plating
    and subculturing
    of fungi
A

Sabouraud dextrose agar

20
Q
  • Intact sheep red
    blood cells
A

Sheep blood agar

21
Q
  • Primary plating
    and subculturing
    of most bacterial
    isolates
A

Sheep blood agar

22
Q

provides additional nutrients to support
highly fastidious organisms

A

CHOC agar

23
Q

a process in which bacteria selected for
further testing will be inoculated to other plates

A

Subculturing

24
Q

supports most gram-negative rods,
especially Enterobacteriaceae

A

MAC agar

25
Q

– color change as the pH
of environment decreases; produces pink, dark
pink, or red colonies

A

Lactose fermenters

26
Q

remain clear and
colorless

A

Lactose non-fermenters

27
Q

Growth on SBA and CHOC agar but not on MAC agar is
indicative of a?

A

Gram positive isolate or of a fastidious gram negative bacillus or coccus

28
Q

A reaction caused by enzymatic or toxin activity of
bacteria observed in the SBA medium

A

Hemolysis

29
Q

the passing of bright light through the bottom of the plate
or to determine whether the
organism is hemolytic

A

Transillumination

30
Q

what agar does not display hemolysis?

A

CHOC agar

31
Q

Organisms that are α-hemolytic or β-hemolytic on SBA
usually show what color?

A

green coloration

32
Q

Partial lysing of RBCs in an SBA plate

A

a-hemolysis

33
Q

Complete clearing of erythrocytes in SBA around or
under the colonies because of the complete lysis of
RBCs

A

b-hemolysis

34
Q

produce a wide, deep, clear zone of β-hemolysis

A

group a b-hemolytic streptococci

35
Q

produce a
narrow, diffuse zone of β-hemolysis close to the colony

A

group b b-hemolytic streptococci

36
Q

generally produce smaller
colonies than gram-negative bacteria

A

gram positive bacteria

37
Q

a hazy blanket of growth on the surface
that extends well beyond the streak lines.

A

swarming

38
Q

produce colonies that are creamy, white with a dull surface, with feet or pedicles

A

Yeast

39
Q

an inherent characteristic of a specific organism
confined generally to the colony

A

colony

40
Q

old sock odor

A

s. aureus

41
Q

fruity or grapelike

A

p. aeruginosa

42
Q

putrid odor

A

p. mirabilis

43
Q

musty or basement

A

haemophilus spp

44
Q

freshy plowed field

A

nocardia spp

45
Q

Comparative examination of colony
morphology of microorganisms growing on various
culture media.

A

plate reading

BACTE 1 (6 decks)

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