Coags articles Flashcards
In vitro assessment of the effect of acidemia on coagulation in dogs
Andrew G. Burton et al, jvecc 2018
Acidemia results in hypocoagulability (lowered MA and alpha angle) but no change to PT or aPTT
Multicenter in vitro thromboelastography and thromboelastometry standardization
jvecc 2018
To determine the comparability of results obtained by different TF-activated TEGs and ROTEMs
The overall degree of variability both within and b/w centers were unacceptably high (CV >10%)
A prospective evaluation of oral Yunnan Baiyao therapy on thromboleastographic parameters in apparently healthy dogs
jvecc 2018
Dogs that received 1 capsule (250mg) PO BID for 1w had increased G, A30 and A60 values and decreased LY30 and LY60.
aka increased clot strength and reducing fibrinolytic activity although still w/in RI
A commercial soy-based phospholipid emulsion accelerates clot formation in normal canine whole blood and induces hemolysis in whole blood from normal and dogs with inflammatory leukograms
Erica L. Behling-Kelly et al jvecc 2018
Soy-based lipid emulsions (intralipid) cause hemolysis that is more severe in blood from dogs with inflammatory leukograms and accelerate clot formation in canine blood
Intralipid breaks down to lysolecithin = have direct hemolytic effect
In vitro hemolysis of stored units of canine packed red blood cells
Rui R. F. Ferreira et al jvecc 2018
Canine pRBC stored for >35d should be tested to ensure <1% hemolysis
-almost 51% units at 36-42d had >1% hemolysis
Ex vivo evaluation of the efficacy of canine fresh-frozen plasma thawed using a microwave plasma defroster
Turner jvecc 2018
MPD effectively thawed plasma quickly and maintained adequate clotting factors + fibrinogen concentrations
None fell below 40%
No evidence of excessively heated plasma (safety)
A pilot study evaluating the effects of prestorage leukoreduction on markers of inflammation in critically ill dogs receiving a blood transfusion.
Luis Bosch Lozano, et al jvecc 2019
total WBC the same at 2h post but incr at 24h post
No other diff b/w inflammatory parameters, survival rate, or acute transfusion rx
A prospective evaluation of oral Yunnan Baiyao therapy on thromboelastographic parameters in apparently healthy cats.
Jeffrey E Patlogar, et al jvecc 2019
1 cap PO BID for 1w
YB caused sig. decr in HCT and RBC count, no changes to TEG
Inaccurate point-of-care blood glucose measurement in a dog with secondary erythrocytosis
Erin R. McQuinn et al jvecc 2020
Erythrocytosis affects handheld glucometers
WB glucose 10-15% lower than plasma BG d/t difference in water content
Corrected PBGM glucose = PGBM glucose + (1.6xPCV -81.3)
Comparison of native and citrated whole blood samples for rapid thromboelastography in Beagles
Hyebin Wang et al jvecc 2020
rapid TEG vs kaolin-activated TEG
- r-TEG decreased meant time to MA
- Native WB is better than citrated WB samples
In vitro effects of lactated Ringer’s solution, hypertonic saline, hydroxyethyl starch, hypertonic saline/hydroxyethyl starch, and mannitol on thromboelastographic variables of canine whole blood
Chan JVECC 2020
Citrated WB diluted with fluids - not infused then collected
All fluid types caused dose-dependent hypercoagulable changes on TEG
Viscoelastic changes also influenced by tonicity and osmolarity
-HTS 7% + mannitol both affected variables more than just dilution (prolonged R+K time, decr alpha angle + MA, incr LY60)
HTS 3.4% have less effect on coag than HTS 7%
Purification of canine albumin by heat denaturation in a plasma bag
Edwards jvecc 2020
Sodium caprylate is able to stabilize canine albumin enabling it to withstand heating that denatures other plasma proteins
Acute hemolytic reaction due to A-B mismatched transfusion in a cat with transient AB blood type
Koenig jvecc 2020
Unknown initial blood type + received untyped matched blood –> developed acute hemolytic transfusion reaction -> immunochromatography blood type suggested AB but crossmatch from donors suggest type B –> gave type B blood, did fine
~Trasient AB type showed that crossmatch should be performed to aid identification
Effect of 7.5% hypertonic saline solution on whole blood coagulation in healthy dogs using thromboelastography
Kim jvecc 2020
No diff in TEG but diff in Na, Cl, osmolality levels b/w 7.5% HTS and salien
Development and assessment of a formal learning module to educate veterinary students in an intensive care unit about transfusion reactions
Haines jvecc 2020
Teaching module can be delivered successfully
Those who took course did better up to 6w after module
The influence of packed cell volume versus plasma proteins on thromboelastographic variables in canine blood
Lynch jvecc 2020
Plasma proteins, not PCV, affected TEG variables – anemic TEG changes are likely related to available fibrinogen
The use of smartphone-obtained images to extrapolate canine packed cell volume
Brehency jvecc 2020
Most accurate in predicting PCV in normal erythrocyte mass or polycythemic samples
Retrospective evaluation of fresh frozen plasma use in 121 cats: 2009–2016
lane jvecc 2020
Coagulopathy, hemorrhage, hypotension Liver dz, cancer Mean 6mL/kg ATR 16%- mild, similar to pRBC rate (elevated temp, tachypnea/dyspnea) Mortality 54%
BP + coagulopathy improved post-transfuison
Incidence of hospital-acquired anemia in hospitalized dogs and cats
Jennie L. Hiratzka javma 2018
Incidence: 34% at 24h and 43% at 48h
(Overall 70% in cats and 43% in dogs)
Increased odds of developing HAA if underwent sx or if cumulative phlebotomy volume >3% of total blood volume
Postoperative thrombocytosis and thromboelastographic evidence of hypercoagulability in dogs undergoing splenectomy for splenic masses
Whitney E. Phipps JAVMA 2020
Thrombocytosis along with TEG hypercoagulability peaked at 7 days but most common during first 2w of sx
(Peri-op mortality rate 7.6%; 22% PST and 10% PTE)
Effect of cross-match on packed cell volume after transfusion of packed red blood cells in transfusion-naïve anemic cats
-AMC paper
Brittany Sylvane jvm 2018
No sig. diff b/w transfusion rx or delta PCV b/w those who had cross-match vs those didn’t in AB typed transfusion
Major cross-match test might not be sensitive enough to detect all pRBC antibody incompatibilities between donor and recipient cats, which precludes its value as a pretransfusion test.
Prevalence of naturally occurring non-AB blood type incompatibilities in cats and influence of crossmatch on transfusion outcomes
-UPENN
Megan E. McClosky jvim 2018
15% cats had alloantibodies outside of AB system
Admin of cross-matched pRBC didn’t change delta PCV
Febrile transfusion rx more common in non-crossmatched blood
Crossmatch didn’t impact outcome @ 30 or 60-d post transfusion
Effects of 6% tetrastarch or lactated Ringer’s solution on blood coagulation in hemorrhaged dogs
Miriely S. Diniz, jvim 2018
Tetrastarch didn’t change primary hemostasis
Both TS + LRS induced transient dilution coagulopathy
Pharmacokinetics of tranexamic acid in healthy dogs and assessment of its antifibrinolytic properties in canine blood
Katie E. Osekavage AJVR 2018
Admin of TXA improved clot strength (incr MA) and decreased fibrinolysis (LY30) in healthy dogs
TXA 15-20 mg/kg PO
Effects of storage over a 36-month period on coagulation factors in a canine plasma product obtained by use of plasmapheresis
Margret E. Donahue DVM AJVR 2019
Plasma collected by plasmapheresis instead of traditional centrifugation
all labile factors remained at > 50% activity for longer than 1 year.
Apheresis may have helped preserve them
Effect of packed red blood cell transfusion on thromboelastographic tracings in dogs with naturally occurring anemia
Aimee C. Brooks AJVR 2020
pRBC transfusion had only small effects on the TEG tracings of hemodynamically stable dogs.
Influence of canine donor plasma hemostatic protein concentration on quality of cryoprecipitate.
Macy Drinkhouse, jvim 2019
No breed difference was found in mean CRYO FV8 content, but vWF and fibrinogen levels lower in greyhounds
cryo from greyhounds still within acceptable limits
Detection of naturally occurring alloantibody by an in-clinic antiglobulin-enhanced and standard crossmatch gel column test in non-transfused domestic shorthair cats.
Isabelle Goy-Thollot, jvim 2019
AB typing using immunochromatographic strip is as accurate as laboratory flow cytometry.
feline antiglobulin-enhanced gel column (AGC) XM tests identified outside of AB incompatibilities
Therapeutic monitoring of rivaroxaban in dogs using thromboelastography and prothrombin time.
Junwoo Bae jvim 2019
Therapeutic Anti-Xa in dogs correlated to 1.5-1.9x PT and T values of TEG 3h after rivaroxaban admin
Primary hemostatic function in dogs with acute kidney injury.
Duana McBride jvim 2019
AKI group had lower PCV, higher plt, lower plt aggregatory, and higher vWF:collagen binding activity
AKI = decreased collagen-activated platelet aggregation and appear to have a type II von Willebrand disease-like phenotype
BMBT = not an appropriate test in AKI
Alloimmunization of a dog erythrocyte antigen 1- dog transfused with weakly dog erythrocyte antigen 1+ blood.
Maryline Guidetti, jvim 2019
Strong and durable (16-1657d) alloimmunization occurs when DEA 1- dog receives blood from weak DEA 1+ dog
Weakly DEA 1+ is still immunogenic
Must crossmatch all previously transfused dog
Neutrophil extracellular traps in stored canine red blood cell units
Erin R. McQuinn jvim 2020
More NETs in non-LR units but can be attenuated by LR before storage
