Chromatography

Question 1

Chromatography

Answer 1

Set of lab techniques that are used to separate the components of mixtures

Question 2

Mikhail Tsvet

Answer 2

Developed chromatography by separating plant pigments

Question 3

Adsorption

Answer 3

Molecules interact with stationary phase at its surface

Question 4

Partition coefficient

Answer 4

Describes the distribution of a solute between the stationary and mobile phases

Question 5

Column chromatography

Answer 5

Mixture flows through a column of adsorbent in powder form

Question 6

Paper chromatography

Answer 6

Cellulose adsorbent paper is the stationary phase, the rate of movement of mobile phase depends on interaction with hydroxyl group in cellulose

Question 7

Retardation factor

Answer 7

If value is 1, molecule is too absorbed into m phase
If value is 0, molecule is stuck to stationary phase

Question 8

Thin layer chromatography

Answer 8

Solvent mixture passes thro thin film of adsorbent solid, silica, coated onto a glass plate

Question 9

Gas chromatography

Answer 9

Stationary phase is adsorbent material impregnated with a liquid, mobile phase is gas carrying the vapors of volatile mixtures

Question 10

Detection for gas chromatography

Answer 10

Flame ionization detector and electron capture

Question 11

High performance liquid chromatography

Answer 11

Solution mixture pumped through a packed column at high pressure

Question 12

HPLC detection

Answer 12

Uv detector with a flow cell and mass detector

Question 13

Retention time

Answer 13

Time taken for compound to appear as a peak

Question 14

Elution time

Answer 14

Time taken to emerge from the column

Question 15

What does separation depend on?

Answer 15

Mobile phase flow rate and solvent quality

Question 16

Ion exchange chromatography

Answer 16

Separation occurs based on charge, the stationary phase is composed of charged particles

Question 17

Size exclusion chromatography

Answer 17

Stationary phase is porous particles, the small molecules that get stuck to them are removed by adding a buffer

Question 18

Affinity chromatography

Answer 18

Stationary phase is matrix covalently coupled to molecule that specifically interacts with the protein of interest, protein with the lower affinity gets separated first, the other is released by ph change or concentrated salt solutions

Question 19

Factors affecting how long substance stays in column

Answer 19

Stationary phase, mobile phase, degree of affinity to both phases and temperature