Characteristics of Chromatographic Separation

Question 1

What is the 1st characteristic?

Answer 1

Retention

Question 2

What is retention directly proportional to?

Answer 2

Flow rate of mobile phase
= slower the flow rate = more stuff retained

Question 3

What is the 2nd characteristic?

Answer 3

Efficiency

Question 4

Describe efficiency

Answer 4

Narrower the peaks, the more efficient the separation

Question 5

Why is a lower, wider peak have lower efficiency?

Answer 5

Can hide material = other components

Question 6

What is the 3rd characteristic?

Answer 6

Selectivity

Question 7

If the selectivity is 1, what does it mean?

Answer 7

Separation of 2 components is impossible

Question 8

What does high selectivity look like?

Answer 8

The peaks are far apart

Question 9

What is the 4th characteristic?

Answer 9

Peak symmetry

Question 10

What are the values for perfect symmetry?

Answer 10

1.0-1.05

Question 11

What has the biggest impact on peak symmetry?

Answer 11

Stationary phase

Question 12

What is the 5th characteristic?

Answer 12

Resolution

Question 13

What is resolution?

Answer 13

How well 2 elution peaks can be differentiated

Question 14

What is the a value for a good resolution?

Answer 14

1.50