chapters 6-9 Flashcards
light microscopy
used to detect small objects, most well used tool in research
electron microscopy
scanning electron microscopy, transmission electron microscopy
prokaryotic
domain bacteria, domain archaea
eukaryotic
protist (multiple kingdoms), kingdom plantae, kingdom fungi, kingdom animilia
prokaryotic characteristic
no nucleus, single circular strand of DNA, no membrane bound organelles
both pro/eukaryotic characteristics
cytoplasm, plasma membrane, chromosomes (carry genes), ribosomes (make proteins
eukaryotic
have nucleus, multi strand DNA, Bigger, phospholipids make membrane, organelles wrapped in membrane
surface to volume ratio
as the cell gets bigger, the surface area to volume ratio gets smaller, if a cell gets to big, not enough material will be able to pass through the membrane
amino acid r groups
r groups changes the chemical properties of each amino acid, 20 different R groups - amino acid
phospholipids are made up of…
phosphate group, glycerol, hydrophilic head, hydrophobic tails
fluid mosaic model
proteins move laterally within the membrane, made up of phospholipids, protein, glycoliids, glycoproteins, lipoproteins, cholesterol
central dogma
dna is transcribed into mRNA, then mRNA is translated into proteins
transcription occurs in the…
nucleus
translation occurs in the…
cytoplasm
ribosomes
make proteins
endomembrane system
rRNA exits through the nuclear envelope to the rough ER, if needed it continues to the smooth ER, if not goes to golgi apparatus (cis>trans), then lysosomes to be broken down again or stored
smooth endoplasm recticlum
no ribosomes, lipid synthesis, detoxification of drugs and alc, storage of calcium ions, released during muscle cell contractions
rough endoplasmic reticulum
studded with ribosomes, distributes proteins, make more membrane ER enzymes, distribute to other portions of system, protein modif, folding and add sugar molecules
protein secretion
ribosomes, to polypeptide, glycoproteins, transport vesicles bud off, into a sugar chain
golgi apparatus
additional modification of protein synthesized in the Er, receiving side: cis-golgi, shipping side: trans-golgi
lysosomes
acidic membranous sac of digestive enzymes “breakdown body”, enzymes synthesized in ER and modified in golgi
phagocytosis
cell engulfs a solid particle
autophagy
lysosomes breaking down damaged organelle
vacuoles
largest vesicles derived from ER or golgi
food vacuoles, contractile vacuole, storage of organic molecules
peroxisome
bound by a single membrane, not part of endomembrane system
Function: contains enzymes that detoxify peroxide to H2O, metabolize fatty acids
metabolic pathway
a series of chemical reactions that either builds or breaks down complex molecules
anabolic
used energy to construct macromolecules
catabolic
break down of organic matter to harvest energy
metabolism path
starts as theronine in active site, end product is isoleucine
mitochondria
catabolic metabolism, breakdown sugars and macromolecules to yield HTP
chloroplasts
anabolic metabolism, generate sugar from light energy
endosymbiant theory
explains how eukaryotic cells may have evolved from prokaryotic cells. Symbiosis is a close relationship between two different organisms. … Later, a host cell engulfed a prokaryotic cell capable of photosynthesis. This is where the chloroplast and other plastids originated.
cytoskeleton
protein fibers that extend throughout the cytoplasm, provide structural support and cell morality
what makes up cytoskeleton
microfilaments, intermediate filaments, microtubules
microfilaments
composed of actin monomers, thinnest of cytoskeleton fibers, arranged in 3D network just inside cell membrane, provides structure for sells that lack cell wall. interact with myosin to create contraction in muscle cells and amoeboid movement in cells
intermediate filament
composed of various fibrous proteins supercoil into cable like structures, permanent structures, reinforce shape, support anchors organelles
microtubules
composed of tubulin dimers, thickest of cytoskeletal fibers, provide track for intracellular transport, guide movement of chromosomes during cellular div., composed of cilia and flagella
cilia
short appendages, typically many per cell, found in protist and multicellular organisms
flagellum
long appendages, typically 1 per cell, found in protist, plants, and multicellular organisms
tight junction (animal tissues)
cell membranes tightly knit together by proteins
anchoring junction (animal tissues)
connection btw cells composed of keratin intermediate filaments
gap junction (animal tissues)
channels that allow molecules to pass btw cells, comm. and coordination
plant cell walls
composed of fibers of cellulose embedded in a matrix of other polysaccharides and protein
plasmodesmata
channels btw adjacent plant cells, allow molecules to pass btw cells, share H2O, nourishment and chemical messages
phospholipid
membranes form spontaneously from phospholipids, made up of phosphate group, glycerol, hydrophilic head, hydrophobic tail, macromolecule polarity influences function (phospholipid, amino acids)
frye and edidin expirament
membrane proteins (mice cell&human cell) come together into a hybrid cell after 1 hour
cholesterol, unsaturated vs saturated carbon tails
unsaturated tails prevent packing (fluid) saturated tails pack together (viscous)
cholesterol within animal cell membrane
cholesterol reduces membrane fluidity at moderate temps, but at low temps hinders solidification
major functions of membrane proteins
transport, enzymatic activity, signal transduction, cell- cell recognition, intercellular joining, attachment to cytoskeleton and extra cellular matrix
human immunodeficiency virus
signal transduction protein in order to infect someone must bind to 2 receptors (CD4, CCR5) if there’s not both, won’t be infected
fibers of extracellular matrix
attachment to cytoskeleton and extracellular matrix
signaling molecules and receptors
signal transduction