Chapter 9: Cell Imaging

Question 1

Whats the approximate size of a standard eukaryote?

Answer 1

30-40 um

Question 2

Whats the size of a red blood cell

Answer 2

~8 um

Question 3

Whats the approximate size of a mitochondria

Answer 3

~2 um

Question 4

Whats the approximate size of a ribosome

Answer 4

~20 nm

Question 5

This is a type of light microscopy that passes incident light through thin, stained cells

Answer 5

“bright-field microscopy”

Question 6

this is a type of light microscopy that only views scattered light rays to produce a negative contrast image

Answer 6

dark-field microscopy

Question 7

this is a type of light microscopy that views unstained (sometimes live) samples by viewing incident light waves out of phase

Answer 7

phase-contrast microscopy

Question 8

This stains nucleic acids (i.e. chromosomes) a dark blue/purple

Answer 8

Hemotoxylin

Question 9

This stains cytoplasmic portions of a cell pink

Answer 9

Eosin

Question 10

What are the 3 filters of fluorescent microscopy?

Answer 10

1. 1st barrier filter
2. beam splitting mirror
3. 2nd barrier filter

Question 11

This is a filter used in fluorescent microscopy that isolates blue light to excite electrons to emit green light

Answer 11

1st barrier filter

Question 12

This is a portion of a fluorescent microscope that reflects light @ <510 nm and transmits light @ >510 nm

Answer 12

beam splitting mirror

Question 13

This is a portion of a fluorescent microscope that isolates specific green pigment emission between 520-560 nm

Answer 13

2nd barrier filter

Question 14

This type of microscopy uses mirror optics to reflect light through a specimen at a fairly large scale

Answer 14

light microscopy

Question 15

this type of microscopy uses electrical field optics to pass electrons through a specimen at a smaller scale

Answer 15

electron microscopy

Question 16

which type of microscopy typically has greater levels of resolution?

Answer 16

electron microscopy

Question 17

This type of electron microscopy is typically used to view a very small specimen to produce flat images (similar to bright-field)

Answer 17

TEM (Transmission electron microscopy)

Question 18

This type of electron microscopy is typically used to view ‘larger’ specimens by reflecting e- off of gold sputter (similar to dissecting microscopy)

Answer 18

SEM (Scanning electron microscopy)

Question 19

A type of fluorescent stain sourced from bioluminescent jellyfish that can be used to form ‘chimeric’ proteins by fusing at the genetic level

Answer 19

GFP (Green Fluorescent Protein)

Question 20

lower magnification tends to yield what?

Answer 20

deeper focal view