Chapter 6 Microbial Growth and Requirements

Question 1

Requirements for Microbial Growth

Answer 1

Physical

Chemical

Question 2

Temp
pH
Osmotic pressure
Barometric pressure

Answer 2

physical requirements for growth

Question 3

Carbon
nitrogen
sulfur
phosphorous
Trace elements 
oxygen
organic growth factor

Answer 3

chemical requirements for growth

Question 4

(Physical Requirements) Temperature

Answer 4

Minimum growth temp
Optimum growth temp
Maximum growth temp

Question 5

(Physical Requirements) pH

Answer 5

growth happens at pH of 6.5-7.5
molds and yeast grow at pH of 5 and 6
Acidophiles grow in acidic enviorments

Question 6

(Physical Requirements) Osmotic pressure

Answer 6

Hypertonic enviorments, increase in salt or sugar causes plasmolysis
Extreme or obligate halophiles require high osmotic pressure
Facultative halophiles tolerate high osmotic pressure

Question 7

_________ are microbial communities that form slime or hydrogels

Answer 7

biofilms

Question 8

Patients with biofilms in catheters received contaminated ________?

Answer 8

heparin

Question 9

Pseudomonas fluorescens appears what color on an agar?

Answer 9

green

Question 10

__________ is nutrients prepared for microbial growth

Answer 10

culture medium

Question 11

no living microbes

Answer 11

sterile

Question 12

introduction of microbes to medium

Answer 12

inoculum

Question 13

microbes growing in or on culture medium

Answer 13

culture

Question 14

_______ is a complex polysaccharide

Answer 14

agar

Question 15

an ______ liquiefies at 100c and solidifies 40c

Answer 15

agar

Question 16

Chemically defined media?

Answer 16

exact chemical composition is known

Question 17

complex media?

Answer 17

extracts of digests of yeasts, meat, or plants

agar or broth

Question 18

*microbial growth on agar will look different bases on metabolism

Answer 18

differential agar/broth

Question 19

*ingredient in agar that most microorganisms cannot tolerate

Answer 19

selective agar/broth

Question 20

Requirements for growth

Answer 20

environment

nutrients

Question 21

differential media is?

Answer 21

agar/broth- microbial growth will appear different based on metablosim

Question 22

selective media is?

Answer 22

agar/broth- ingredient included that most microorganisms cannot tolerate

Question 23

a closed system is?

Answer 23

starts with lag, deceleration occurs, equilibrium, then cell microbe death
no added food or waste removal

Question 24

a open system is?

Answer 24

lag, lag, and continuous numbers of microbes.

addition of food and removal of waste

Question 25

what are methods for counting microbes?

Answer 25

direct

indirect

Question 26

________ counting counts biproducts of metabolism or turbidity

Answer 26

indirect

Question 27

turbidity is used most for ______ to find ______ that contains microbes

Answer 27

water, dirt

Question 28

_______ counting counts cells and colonies by membrane filtration and serial dilution

Answer 28

direct