Chapter 6&7 word list Flashcards
focus a beam of electrons onto the surface of a specimen, providing images that look 3D
Scanning electron microscope
- visible light passes through a specimen and then through glass lenses, which magnify the image
- The minimum resolution of an ____ _____ is about 200 nanometers (nm), the site of a small bacterium
- can magnify effectively to about 1,000 times the size of the actual specimen
- Various techniques enhance control and enable cell components to be stained or labeled
- Most subcellular structures, or organelles, are too small to be resolved by a LM
Light Microscope
Scientists use ____ to visualize cells too small to see with the naked eye
microscope
focus a beam of electrons through a specimen
Transmission electron microscopes
subcellular structures
organelle
- takes cells apart and separates the major organelles from one another
- Ultracentrifuges fractionate cells into their component parts
- Enables scientists to determine the functions of organelles
Cell fractionation
semifluid substance called the _____ (or cytoplasm)
Cytosol
What are the basic features of ALL cells
- Plasma membrane
- Semi Fluid substance called the cytosol (or cytoplasm)
- Chromosomes (carry genes)
- Ribosomes (Make proteins)
- Have no nucleus
- DNA is in an unbound region called the nucleoid
- lack membrane bound organelles
Prokaryotic Cells
- DNA in a nucleus that is bounded by a membranous nuclear envelope
- have membrane bound organelles
- generally much larger
- The logistics of carrying out cellular metabolism sets limits on the size of cells
Eukaryotic cells
- a selective barrier that allows sufficient passage of oxygen, nutrients, and waste to service the volume of the cell.
- general structure of a biological membrane is a double layer of phospholipids
Plasma membrane
- genetic library of the cell
- contains most of the DNA in a eukaryotic cell
- Ribosomes use the information from the DNA to make proteins
- contains most of the cell’s genes and is usually the most conspicuous organelle
- the nuclear envelope encloses the ____, separating it from the cytoplasm
Nucleus
carry genes
chromosomes
non membranous organelle involved in production of ribosomes; a nucleus had one or more nucleoli
nucleolus
-carry out protein synthesis in two locations:
-in the cytosol (free ribosome)
-on the outside of the endoplasmic reticulum(ER)
or the nuclear envelope (bound ribosomes)
-are particles made of ribosomal RNA and rRNA and protein
Ribosome
-regulates protein traffic and performs metabolic functions in the cell
Endomembrane system
What are the components of the endomembrane system
- Nuclear envelope
- Endoplasmic reticulum
- Golgi apparatus
- Lysosomes
- Vacuoles
- Plasma membrane
These components are either continuous or connected via transfer by vesicles
composed of DNA and associated proteins; it carries the cell’s genetic information.
Chromatin
- membrane bound sacs with varied functions
- diverse maintenance compartment
- contains materials in transport of the cell
Vesicles and Vacuoles (larger versions of vesicles)
- accounts for more than half of the total membrane in many eukaryotic cells
- membrane is continuous with the nuclear envelope
Endoplasmic reticulum
- biosynthetic factory
- ribosomes studding its surface
- has bound ribosomes
- Produces proteins and membranes, which are distributed by transport vesicles
- membrane factory for the cell
Rough ER
- biosynthetic factory
- synthesizes lipids
- metabolizes carbohydrates
- stores calcium
- detoxifies poison
Smooth ER
-shipping and receiving center
-Consists of flattened membranous sacs called cisternae
Functions: Modifies products of the ER,
Manufactures certain macromolecules, sorts and packages materials into transport vesicles
Golgi Apparatus
-digestive compartments
-membranous sac of hydrolytic enzymes (=”recycling center”)
-can hydrolyze proteins, fats, polysaccharides,
and nucleic acids
-use enzymes to recycle organelles and macromolecules, a process called autophagy
Lysosomes
formed by phagocytosis
Food Vacuoles
found in many freshwater protists, pump excess water out of cells
Contractile Vacuoles
found in many mature plant cells, hold organic compounds and water
Central Vacuoles(plant cells)
Chemical energy conversion
- in nearly all eukaryotic cells
- have a smooth outer membrane and an inner membrane folded into cristae (crinkled cristae)
- the inner membrane creates two compartments: intermembrane space and mitochondrial matrix (Mito Middle=Matrix)
- some metabolic steps of cellular respiration are catalyzed in the _____ matrix
- Cristae present a large surface area for enzymes that synthesize ATP
Mitochondria: Chemical energy conversion
present a large surface area for enzymes that synthesize ATP
Cristae
-member of a family of organelles called plastids
-contain the green pigment chlorophyll, as well as enzymes and other molecules that function in photosynthesis
-found in leaves and other green organs of plants and algae
structure includes: Thylakoids, membranous sacs
Stroma, the internal fluid
Chloroplast: Capture of light energy
membranous sacs
Thylakoid
internal fluid
Stroma
- specialized metabolic compartments bounded by a single membrane
- contain the enzyme catalase to convert the hydrogen peroxide to water
Peroxisome
a network of fibers that organizes structures and activities in the cell
- a network of fibers extending throughout the cytoplasm
- organizes the cell’s structures and activities, anchoring many organelles
- composed of three types of molecular structures:
- Microtubules
- Microfilaments
- Intermediate filaments
Cytoskeleton: Support, motility, Regulation
helps to support the cell and maintain its shape
- interacts with motor proteins to produce motility
- inside the cell, vesicles can travel along the “monorails” provided by the _____
- may help regulate biochemical activities
Cytoskeleton: Support, Motility, Regulation
Protein Subunits: Tubulin, consisting of a tubulin and b tubulin
Functions:
-Shaping the cell
-Guiding movement of organelles
-Separating chromosomes during cell division
-control the beating of cilia and flagella, locomotor appendages of some cells
Microtubule
Protein subunits: Actin
Main functions: Maintenance of cell shape (tension-bearing elements)
-Changes in cell shape
-Muscle contraction
-Cytoplasmic streaming
-Cell motility
-Cell division (cleavage furrow formation)
Microfilaments (Actin Filaments)
Protein subunits: One of several different proteins of the keratin family; depending on cell type
Main functions: Maintenance of cell shape (tension-bearing elements)
-Anchorage of nucleus and certain other organelles
-Formation of nuclear lamina
Intermediate Filaments
“microtubule organizing center”
1) in many cells, microtubules grow out from a ____ near the nucleus
2) In Animal cells, the ____ has a pair of ____, each with nine pairs or triplets of microtubules arranged in a ring
1) centrosome
2) centrosome, centrioles
anchors the cilium or flagellum
Basal body
(cellular extensions) extend and contract through the reversible assembly and contraction of actin subunits into microfilaments
Pseudopodia
- a circular flow of cytoplasm within cells
- This streaming speeds distribution of materials within the cell
- In plant cells, actin-myosin interactions and sol-gel transformations drive _____
Cytoplasmic streaming