Chapter 5/8: Cultivation and Quantification of Viruses

Question 1

What type of assay is used to cultivate lytic bacteriophage?

Answer 1

Plaque Assay

Question 2

What must be used to grow the targets for the viruses?

Answer 2

Tissue culture of susceptible host cells

Question 3

What is an example of a tissue culture used to grow the targets for the viruses?

Answer 3

HeLa cells

Question 4

What can be observed in virally infected cells, indicating damage and impending cell death?

Answer 4

Cytopathic effects (CPE)

Question 5

What term describes the fusion of cells to create a massive multi-nucleated structure in viral infection?

Answer 5

Syncytium

Question 6

What method of virus quantification allows for visualization but doesn’t differentiate between infectious and non-infectious virions?

Answer 6

Direct Count (Electron microscopy)

Question 7

What method of virus quantification allows for determination of titer of original suspension?

Answer 7

Plaque Assay