chapter 4 plant physiology Flashcards
Transport of CO2 and O2 in the leaves
- CO2 enters through the leaves for photosynthesis
- O2 leaves through the leaves as a product of photosynthesis
Transport of CO2 and O2 in the roots
- CO2 leaves the roots as a product of metabolism/ cellular respiration
- O2 enter the roots to be used in cellular respiration
Transpiration
- the movement of water and mineral nutrients from soil to the atmosphere via plants
- water will evaporate from leaf cells at <100% RH
How much water is moved through transpiration per day?
- 0.5 gallons/day in one corn plant
- 52 gallons/day in one large maple
- transpiration is not seen
Evapotranspiration
- landscape level movement of water from soil to atmosphere
Where does water enter the plant?
- water enters through the fine roots
- these are found in the first couple inches in the soil
- the soil surface is significant
- deeper roots are for support
- water travels from the fine roots to the xylem
What controls transpiration?
- controlled by guard cells of the stomata
- turgid guard cells open the stoma
Why are stoma generally open in the day and closed at night?
- photosynthesis occurs with sunlight
- being open allows CO2 to get in but also allows loss of water
- closed at night to prevent excess water loss through stoma and transpiration
What are xerophytes?
- dry plants that often have CAM photosynthesis
- close stomates during the day
How does water move in and out of guard cells?
- overall controlled by K+
- more K+ in a cell = water will move into cell because of gradient
- when guard cells are turgid they open the stoma
Why does increase in turgor pressure open the stomate?
- cellulose microfibrils of guard cells expand in length when hydrated
- because they are surrounded by other cells they elongate as much as they can and create the stromal opening
What are leaf adaptations to growing in arid environments?
- epicuticle and several layers of epidermis
- sunken stomates with hair coverings
- sunken stomates and hairs help keep dry wind from causing excess water loss while still allowing CO2 in
Types of water movement in plants
- apoplastic: water enters through cellulose that surrounds cortical cells, allows water to move more readily
- symplastic: water enters through cortical cells and moves from cell to cell, water is moved less readily
Transpiration-cohesion theory
- water has cohesive and adhesive properties
- cohesive: water can stick to other water molecules via H bonding
- adhesive: water can stick to the cell wall in dead xylem cells
How does water have cohesive and adhesive properties?
- the H2O molecule has both a partially positive and partially negative charge
- this allows H bonding between water molecules
- cellulose also has partial charges so it can form H bonds with water
What is water potential
- water’s free energy or its ability to do work
- composed of solute potential and pressure potential
- solute potential + pressure potential = water potential
Solute potential
- the effect of dissolved solutes on water potential
- always less than or equal to zero
- water moves toward more solute
- increase in solute [ ] decreases the solute potential (makes it more negative)
- add more solute = solute potential is more negative
- if no solute present, solute potential is 0
- cannot be positive
Pressure potential
- effect of hydrostatic pressure of water potential
- most cells have a + pressure potential also called turgor pressure
- functional xylem cells have - pressure potential also called tension
What is significant about the pressure potential of functioning xylem cells?
- they have a negative pressure potential or tension
- water is under tension and because of H bonding properties water can be pulled up the xylem
Which way does water move?
- water always moves to the more negative water potential
- or toward the lower free energy
Movement of water in a plant
- soil, root epidermal cell, parenchyma cell, functional xylem cell, leaf mesophyll (parenchyma) cell, atmosphere
How does water move up the plant?
- the total water potential is more negative as you move up the plant
- water flows to the more negative water potential
- ex. root epidermal cells have a -0.1 MPa water potential and the soil has a 0 MPA water potential, so water moves from the soil to root epidermal cells
What energy is used to transport this water?
- the flow of water to a lower free energy
- no ATP used
What are the effects of soil salinity on soil and plant water potential?
- solute potential of soil becomes more negative and so its water potential becomes more negative
- if it gets too negative, the water won’t be able to get into the plant
Why do parenchymal cells have a more negative solute potential?
- leaf parenchyma produces sugars from photosynthesis and store them, so they have more solute
- stem parenchyma also stores carbs which makes the solute potential more negative
- leaf parenchyma has more sugars, so it is more negative
What are the major essential nutrients for plants?
- nitrogen
- phosphorous
- potassium
- magnesium
- sulfur
- calcium
Importance of nitrogen for plants
- needed to make proteins, aa, and chlorophyll
- 4 N molecules needed per chlorophyll molecule to chelate the magnesium
Importance of phosphorous for plants
- needed to make ATP, nucleic acids, and phospholipids
- phospholipids are important for membranes
Importance of potassium for plants
- needed for guard cell regulation
- more in guard cell = stomates open
Importance of magnesium for plants
- component of chlorophyll
- used by various enzymes
Importance of sulfur in plants
- needed to make proteins and aa
Importance of calcium in plants
- component of the cell wall
Other minerals used by plants
- boron, chlorine, manganese, iron, nickel, copper, zinc, Mo: absorbed from soil and can act as cofactors for some enzymes
- hydrogen, carbon, and oxygen
How do plants get H, C, and O?
- carbon is from CO2 and photosynthesis in leaves
- oxygen and hydrogen from water through the roots
What are benefits of hydroponicproduction?
- less weight
- higher value of crop over winter
- better pest control
- better cost efficiency in the long run
How were essential plant nutrients discovered?
- plants were grown in lab
- some lacked certain nutrients in water
- if a nutrient was missing and caused abnormal growth it was considered an essential mineral
What is phytoremediation?
- the use of plants to clean up contamination from soils, sediments, and water
- works because plants absorb nutrients from the soil even if they are not necessary for them
- takes advantage of the unique and selective uptake capabilities of plant root systems
- environmentally friendly and potentially cost effective
Translocation of sugars
- occurs in LIVING phloem sieve tube members
- requires ATP (chemical energy)
- moves sucrose form source to sink
- described by the pressure flow hypothesis
What are the source and sink in the translocation of sugars?
- source: any part of the plant that is photosynthetic and produces sugars like the leaves or some stems
- sink: non-photosynthetic areas like the roots, storage areas, and some stems
Explain the pressure flow hypothesis
- in the source: sucrose is actively transported (uses ATP) into cells to increase the [] inside the cell, because of this water also moves into the cell
- movement of water into the source cells increases and creates pressure to move the sucrose and water
- water moves through sieve tube members until it reaches the sink cell
- depending on the [ ] of sucrose in the area of the sink cell, sucrose moves out of the cell via active transport or down its gradient - both require and transport protein
- even though water is moved in this process this is NOT the main transport of water in plants
How does water and mineral nutrient transport differ from sugar transport in vascular plants?
- water and minerals are transported via xylem
- xylem cells are dead at function
- sugars are transported via phloem
- phloem is alive at function
- movement of water does NOT require an energy source
- movement of sugars does require and energy source (ATP)
Sugar and Slavery
What two plants provide virtually all the commercial sucrose worldwide?
- sugar cane and sugar beet
- led to the triangular trade from the 1500s-1800s
- britain, west africa, and the west indies
What was traded in the triangular trade?
- britain received rum and sugar from the west indies & shipped firearms, cloth and salt to west africa
- west africa recieved from britain & sent salves to the west indies
- the west indies received from west africa & shipped rum and sugar to britain
Metabolism
- cellular respiration processes
- glucose broken down to create energy
- oxygen is the final electron acceptor and becomes water
Photosynthesis
- plants use light energy from the visible spectrum for photosynthesis
What is the visible spectrum
- wavelengths from 400-700
- 40% of the radiant energy from the sun
Photosynthetic pigments and green plants
- primary pigments: donate electrons in photosynthesis
- accessory pigments: part of light harvesting system
What are the primary pigments in photosynthesis?
- chlorophyll 680 (PS2)
- chlorophyll 700 (PS1)
What are the accessory pigments in photosynthesis?
- the rest of the chlorophyll a’s
- chl b
- carotenoids: carotenes (orange) and xanthophylls (yellow)
- form pigment/protein complexes in the thylakoid membrane
- transfer light to primary chl a so it can donate the electrons
Chlorophyll structure
- amphipathic
- hydrophobic ring with Mg2+ and 4 N molecules that chelate the Mg2+
- hydrophobic hydrocarbon tail that allows chl to imbed into the membrane
- chl a: R group is CH3
- chl b: R group is a CHO
Carotenoid structure
- mainly hydrophobic hydrocarbons that imbed into the membrane
Are carotenoids or chlorophylls broken down in the fall?
- chlorophylls are broken down in the fall
- longer nights during the fall trigger chl breakdown
Why is chl broken down rather than carotenoids?
- chl is made of Mg2+ and 4 N molecules
- N is the most limited essential nutrient for plants
- so chl is broken down so the plant can keep and store the Mg2+ and N until spring
- carotenoids made mostly of carbons and lost with leaves falling but C is easier for plants to get and make more when needed
How is N the most limited mineral for plants to get if it is prominent in the atm?
- the N in the atm cannot be absorbed and fixed by plants
- it is absorbed by bacteria and fixed and released into the soil
- BUT when N is fixed it is mainly made into nitrate and nitrite
- nitrate and nitrite are negatively charged so they do no bind well to soil elements and leak OUT of the soil
- continual leaking from the soil prevents or makes it harder for plants to absorb N
Absorption spectrum details
- chls absorb blues, purples, and reds but reflect greens
- carotenoids absorb green and reds but reflect yellows
- blues and reds spectrum primarily drive photosynthesis
Photosynthesis phases
- light reactions: photochemical phase
- occur on the thylakoid membrane
- convert light to ATP and NADPH - Calvin cycle: biochemical phase
- occurs in the stroma
- converts CO2 into sugars and eventually sucrose with the use of ATP and NADPH
Order of electron movement in photosynthesis light dependent reactions
- PSII (photosystem 2) w chl a 680
- plastoquinone
- cyt b6f complex
- plastocyanin
- PSI w chl a 700
- ferredoxin
- ferredoxin-NADPH reductase
Significance of light reactions?
- occur in the thylakoid membrane
- movement of electrons creates a H gradient in the lumen of chloroplasts
- ferredoxin-NADPH reductase reduces an NADP molecule to NADPH
- ATP synthase uses the H gradient made to synthesize ATP
- ATP and NADPH are made into the stroma and needed for the Calvin cycle that also occurs in the stroma
Why is NADPH significant?
- it is made from the light reactions
- it is the electron source that is needed in the calvin cycle
Ph difference of the lumen and stroma
- lumen: 5
- stroma: 8
- lumen has 1000x more H+ making it much more acidic