Chapter 4 Lecture

Question 1

Transfection

Answer 1

Leads to the discovery of oncogenes

Question 2

Transfection

Answer 2

1. Used to detect oncogenes in the DNA of cancer cells
2. DNA from chemically transformed cells transfected into NIH 3T3 become transformed

Question 3

Process of Transfection

Answer 3

1. Chemically transformed mouse fibroblasts
2. Transfection using calcium phosphate co-precipitation procedure
3. On normal mouse fibroblasts
4. Formation of a focus of morphologically transformed cells
5. Injection of morphologically transformed cells into mouse host
6. Tumor forms