Chapter 3 - Cellular level (Pt2) Flashcards

Discuss the components of the cytoplasm Compare and contrast mitosis and meiosis Understand the effects aging has on the cell

1
Q

Q: What are the two components of the cytoplasm?

A

A: The cytoplasm consists of the cytosol and organelles.

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2
Q

Q: What is the cytosol?

A

A: The cytosol, or intracellular fluid, makes up about 55% of the cell’s volume and is primarily composed of water, ions, nutrients, proteins, lipids, ATP, and waste products.

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3
Q

Q: What percentage of the cytosol is water?

A

A: The cytosol is 75-90% water.

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4
Q

Q: What key process occurs in the cytosol?

A

A: Glycolysis, the breakdown of glucose to produce ATP, occurs in the cytosol.

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5
Q

Q: What is the cytoskeleton?

A

A: The cytoskeleton is a network of protein filaments in the cytosol that maintains cell shape and supports organelles.

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6
Q

Q: What are the components of the cytoskeleton?

A

A: The cytoskeleton is composed of microfilaments, intermediate filaments, and microtubules.

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7
Q

Q: What are microfilaments, and what do they do?

A

A: Microfilaments are the thinnest components, made of actin and myosin, providing mechanical support and enabling movement like muscle contraction and cell division.

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8
Q

Q: What roles do intermediate filaments play in the cell?

A

A: Intermediate filaments provide structural reinforcement, hold organelles in place, and help shape the cell.

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9
Q

Q: What are microtubules, and what are their primary functions?

A

A: Microtubules are cylindrical filaments made of tubulin, providing support, structure, and enabling transportation within the cell.

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10
Q

Q: What are organelles?

A

A: Organelles are specialized structures within cells that have unique shapes and functions to support growth, maintenance, and reproduction.

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11
Q

Q: Why do different cellular reactions occur in separate organelles?

A

A: To prevent interference between reactions and to compartmentalize processes, allowing for specific conditions and enzymes to be maintained.

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12
Q

Q: What determines the type and number of organelles in a cell?

A

A: The cell’s specific role or function determines the type and number of organelles it contains.

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13
Q

Q: How do organelles contribute to homeostasis within a cell?

A

A: While they have distinct functions, organelles work together to maintain balance and proper functioning within the cell.

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14
Q

Q: Why is the nucleus often discussed separately from other organelles?

A

A: Because the nucleus controls the cell’s activities and contains the genetic material.

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15
Q

Q: What is a key feature of organelles in terms of cell processes?

A

A: Each organelle contains specific enzymes for their tasks, acting as compartments for certain processes.

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16
Q

Q: How do organelles contribute to cellular efficiency?

A

A: By compartmentalizing processes, organelles allow for specialized environments and efficient reaction conditions tailored to specific tasks.

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17
Q

Q: What is the primary function of the cytoskeleton?

A

A: The cytoskeleton helps determine cell shape and organizes organelles, aiding in movement, absorption, and support.

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18
Q

Q: What are the three types of filaments in the cytoskeleton?

A

A: Microfilaments, intermediate filaments, and microtubules.

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19
Q

Q: What are microfilaments made of, and what is their function?

A

A: Microfilaments are made of actin and myosin; they aid in movement such as muscle contraction and cell division and provide support.

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20
Q

Q: Where are microfilaments primarily located, and what do they support?

A

A: They are found at the cell’s edge and support cell extensions like microvilli for absorption.

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21
Q

Q: What is the role of intermediate filaments in the cell?

A

A: Intermediate filaments provide strength, hold organelles like the nucleus in place, and help cells attach to each other.

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22
Q

Q: How are microtubules formed, and what are their functions?

A

A: Microtubules are formed from tubulin at the centrosome; they determine cell shape and assist in moving organelles, chromosomes, and structures like cilia and flagella.

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23
Q

Q: What role do microtubules play in cellular movement?

A

A: They assist in the movement of organelles, chromosomes during cell division, and structures like cilia and flagella to facilitate locomotion.

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24
Q

Q: What are cilia?

A

A: Cilia are hairlike projections that extend from the cell surface, helping move fluids along the cell surface.

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25
Q

Q: How do cilia contribute to cell function in the respiratory tract?

A

A: They move mucus and trapped particles away from the lungs to prevent interference with proper cell function.

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26
Q

Q: Describe the movement of cilia.

A

A: Cilia move with a power and recovery stroke, similar to an oar moving through water, which enables them to move fluids over the cell’s surface.

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27
Q

Q: What role do microtubules play in cilia?

A

A: Microtubules are structural components of cilia,.

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28
Q

Q: How are cilia anchored to the cell?

A

A: Cilia are anchored to the cell by basal bodies, which also assist in the assembly of cilia and flagella.

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29
Q

Q: What can impede ciliary action and affect lung function?

A

A: Conditions like cystic fibrosis can cause thick mucus, which hinders ciliary action and affects lung function.

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30
Q

Q: What is the purpose of cilia’s coordinated beating?

A

A: The coordinated beating moves fluids, such as mucus, efficiently across the cell’s surface to clear debris and foreign particles.

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31
Q

Q: What are flagella?

A

A: Flagella are long, whip-like structures similar to cilia but much longer, used to move entire cells.

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32
Q

Q: How do flagella differ from cilia in structure and function?

A

A: Flagella are longer than cilia and primarily function to move entire cells, whereas cilia often move fluids over the cell’s surface.

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33
Q

Q: How does a flagellum move the cell?

A

A: A flagellum moves in a wave-like pattern to propel the cell forward.

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34
Q

Q: What is the only example of a flagellum in human cells?

A

A: The sperm’s tail, which helps it swim toward the egg in the uterine tube.

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35
Q

Q: Describe the motion of a flagellum.

A

A: The flagellum moves by wiggling in a wave-like pattern to push the cell forward.

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36
Q

Q: What is the nucleus, and why is it prominent in the cell?

A

A: The nucleus is a round or oval structure that is usually the most noticeable part of a cell, housing DNA and controlling cell activities.

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37
Q

Q: How do the number of nuclei vary in different cells?

A

A: Most cells have one nucleus, but some, like red blood cells, have none, while others, like muscle cells, have several.

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38
Q

Q: What is the nuclear envelope?

A

A: The nuclear envelope is a double-layered membrane surrounding the nucleus, made of lipid bilayers, with the outer layer connected to the rough ER.

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39
Q

Q: What are nuclear pores, and what is their function?

A

A: Nuclear pores are large openings in the nuclear envelope that control the movement of substances between the nucleus and the cytoplasm.

note: The nuclear envelope is a double-layered membrane surrounding the nucleus, made of lipid bilayers, with the outer layer connected to the rough ER.

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40
Q

Q: How do large molecules like RNA and proteins move through the nuclear pores?

A

A: Large molecules require active transport, moving selectively through nuclear pores with energy input.

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41
Q

Q: What is the nucleolus?

A

A: The nucleolus is a cluster of DNA, RNA, and proteins within the nucleus that produces ribosomes and is prominent in cells synthesizing large amounts of protein, such as skeletal muscle cells.

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42
Q

Q: Why is the nucleolus prominent in certain cells like skeletal muscle cells?

A

A: Because it produces ribosomes, which are crucial for protein synthesis, and these cells have high protein synthesis demands.

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43
Q

Q: What are the hereditary units within the nucleus called?

A

A: The hereditary units are called genes.

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44
Q

Q: How are genes organized in the nucleus?

A

A: Genes are arranged along chromosomes.

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45
Q

Q: What are chromosomes composed of?

A

A: Chromosomes are made up of DNA and a histone protein complex.

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46
Q

Q: What is the role of histone proteins?

A

A: Histone proteins provide structural support for DNA, helping to organize it into chromatin.

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47
Q

Q: What are nucleoli, and what is their function?

A

A: Nucleoli are clusters of proteins, DNA, and RNA in the nucleus that make ribosomal RNA (rRNA) and assemble ribosomal subunits.

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48
Q

Q: In what type of cells are nucleoli especially prominent and why?

A

A: Nucleoli are prominent in cells that produce a lot of protein, such as muscle and liver cells, due to high demands for ribosome production.

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49
Q

Q: What happens to nucleoli during cell division?

A

A: Nucleoli temporarily disappear during cell division and reappear in newly formed cells.

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50
Q

Q: What is chromatin, and how does it appear in non-dividing cells?

A

A: Chromatin is the material that makes up chromosomes, appearing as a loose, granular mass with a “beads-on-a-string” structure in non-dividing cells.

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51
Q

Q: What is a nucleosome?

A

A: A nucleosome consists of DNA wrapped twice around a core of eight histone proteins, forming the “beads” in the chromatin structure.

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52
Q

Q: What is linker DNA?

A

A: Linker DNA is the “string” connecting nucleosomes, maintaining the chromatin structure.

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53
Q

Q: What happens to chromatin during cell division?

A

A: Chromatin fibers condense into chromatids, and each pair of chromatids forms a chromosome during cell division.

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54
Q

Q: How many chromosomes do humans have, and where is this information contained?

A

A: Humans have 46 chromosomes in body cells, with 23 inherited from each parent; this complete set forms the genome.

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55
Q

Q: What is the primary function of ribosomes in the cell?

A

A: Ribosomes are the structures where proteins are made in the cell.

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56
Q

Q: What are ribosomes composed of?

A

A: Ribosomes are made of ribosomal RNA (rRNA) and over 50 proteins.

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57
Q

Q: Describe the structure of a ribosome.

A

A: Each ribosome consists of two subunits: a large one and a smaller one.

58
Q

Q: Where are ribosomal subunits made?

A

A: Ribosomal subunits are made in the nucleolus inside the nucleus.

59
Q

Q: How do ribosomal subunits become functional ribosomes?

A

A: The subunits leave the nucleus separately and combine in the cytoplasm to become functional ribosomes.

60
Q

Q: What are attached ribosomes, and what do they do?

A

A: Attached ribosomes are connected to the nuclear membrane and the endoplasmic reticulum, making proteins for organelles, the cell membrane, or export out of the cell.

61
Q

Q: What are free ribosomes, and where do they operate?

A

A: Free ribosomes float in the cytoplasm and produce proteins that will be used inside the cell.

62
Q

Q: What are mitochondrial ribosomes, and what is their role?

A

A: Mitochondrial ribosomes are found in mitochondria and make proteins for the mitochondria itself.

63
Q

Q: Where can ribosomes be found in the cell?

A

A: Ribosomes can be found in nucleoli, free in the cytosol, and on the rough ER.

64
Q

Q: How do ribosomes assemble proteins?

A

A: Ribosomes use directions in messenger RNA (mRNA) to assemble amino acids into proteins, as specified by the genetic code (DNA).

65
Q

Q: Why are proteins essential for cells?

Hint - Mnemonic: “Happy Tigers Can Hunt Many Energetic Tigers.”

A

Homeostasis maintenance
Trait determination

Cellular structure formation

Hormone and antibody functions

Muscle component formation

Enzyme activity (regulating chemical reactions)

Transport of materials in the blood

66
Q

Q: What is the complete set of proteins in an organism called?

A

A: The complete set of proteins is called the proteome.

67
Q

Q: What is the process of transcription?

A

A: Transcription is the process of copying DNA into RNA.

67
Q

Q: What does gene expression involve?

A

A: Gene expression involves using DNA to make proteins.

68
Q

Q: What occurs during translation?

A

A: During translation, RNA guides the assembly of amino acids into proteins at the ribosome.

69
Q

Q: Where does transcription occur in the cell?

A

A: Transcription occurs in the nucleus.

70
Q

Q: What is the purpose of transcription?

A

A: The purpose of transcription is to copy genetic information from DNA onto messenger RNA (mRNA) to direct protein synthesis.

71
Q

Q: What is a promoter in DNA?

A

A: A promoter is the DNA sequence where transcription begins, signaling RNA polymerase to start making mRNA.

72
Q

Q: What is a terminator in DNA?

A

A: A terminator is the DNA sequence where transcription ends, telling RNA polymerase to stop and release the mRNA.

73
Q

Q: What enzyme is responsible for transcription?

A

A: RNA polymerase is responsible for transcription.

74
Q

Q: Which strand of DNA is used during transcription?

A

A: Only one strand of DNA, the template strand, is used to make RNA.

75
Q

Q: During transcription, what base pairs with adenine in RNA?

A

A: In RNA, uracil (U) pairs with adenine (A).

76
Q

Q: What are exons and introns?

A

A: Exons are sequences that code for proteins, while introns are non-coding sequences located between exons.

77
Q

Q: What happens to pre-mRNA after transcription?

A

A: The introns are removed, and the exons are spliced together by snRNPs to form functional mRNA, which then leaves the nucleus for protein synthesis.

78
Q

Q: What is the role of RNA polymerase during transcription?

A

A: RNA polymerase binds to the promoter, reads the DNA template, and creates a complementary RNA strand until it reaches the terminator.

79
Q

Q: What enzyme catalyzes transcription, and where does it occur?

A

A: RNA polymerase; occurs in the nucleus.

80
Q

Q: What are the three types of RNA produced during transcription, and their functions?

A

A:

mRNA: Directs protein synthesis.
rRNA: Forms ribosomes.
tRNA: Transfers amino acids during protein synthesis.

81
Q

Q: How does the DNA template AGCT convert into mRNA?

A

A: It becomes UCGA in the mRNA.

82
Q

Q: What is the role of the promoter and terminator in transcription?

A

A: The promoter is where transcription begins, and the terminator is where it ends.

83
Q

Q: What are introns and exons, and how are they processed?

A

A: Introns are non-coding regions removed from pre-mRNA, while exons are coding regions spliced together to form functional mRNA.

84
Q

Q: What base does adenine pair with in RNA, and what replaces thymine?

A

A: Adenine pairs with uracil in RNA, and uracil replaces thymine.

85
Q

Q: Where does translation take place?

A

A: Translation occurs in the cytoplasm.

86
Q

Q: What is the role of mRNA in translation?

A

A: mRNA carries the genetic code for specifying the amino acid sequence of a protein.

87
Q

Q: What initiates the translation process?

A

A: The ribosome assembling around the target mRNA and the first tRNA attaching to the start codon.

88
Q

Q: How does the translation process conclude?

A

A: Translation ends when a stop codon is reached, prompting the ribosome to release the completed protein.

89
Q

Q: What does tRNA do during translation?

A

A: tRNA matches amino acids to their corresponding mRNA codons and facilitates their addition to the growing polypeptide chain.

90
Q

Q: Describe the function of the ribosome’s small and large subunits.

A

A: The small subunit binds to mRNA, while the large subunit has three sites (P, A, E) for tRNA binding.

91
Q

Q: What are the functions of the P, A, and E sites on the ribosome?

A

P Site: Holds the tRNA with the growing protein chain.
A Site: Holds the tRNA with the next amino acid to be added.
E Site: Releases tRNA after its amino acid has been added.

92
Q

Q: What are post-translational modifications?

A

A: They are changes that proteins undergo after synthesis during translation, crucial for their final structure, function, and cellular localization.

93
Q

Q: How can post-translational modifications alter a protein’s activity?

A

A: By enabling or disabling specific functional sites, thus impacting its role within the cell.

94
Q

Q: Why are these modifications important for protein localization?

A

A: They direct proteins to their proper cellular compartments, ensuring they function where needed.

95
Q

Q: How do post-translational modifications affect protein interactions?

A

A: They can modulate the affinity or specificity for interacting with other molecules.

96
Q

Q: What role do these modifications play in protein stability?

A

A: They can stabilize proteins for active use or mark them for degradation to regulate lifespan.

97
Q

Q: What is the importance of protein degradation?

A

A: It breaks down damaged or unneeded proteins, maintaining cellular homeostasis.

98
Q

Q: How do post-translational modifications relate to cellular homeostasis?

A

A: They ensure proper protein functionality and timely degradation, balancing cellular processes.

99
Q

Q: What connects the ER to the rest of the cell?

A

A: The ER connects to the nuclear envelope and spreads throughout the cytoplasm.

100
Q

Q: What differentiates rough ER from smooth ER structurally?

A

A: Rough ER has ribosomes attached to its surface, while smooth ER does not.

101
Q

Q: What are the primary functions of the rough ER?

A

A: Protein synthesis, lipid production, and protein processing and sorting.

102
Q

Q: What are the primary functions of the smooth ER?

A

A: Lipid synthesis, detoxification, carbohydrate metabolism, and calcium storage.

103
Q

Q: What specific types of lipids are produced by the smooth ER?

A

A: Fatty acids, and steroids such as estrogen and testosterone.

104
Q

Q: How does the rough ER contribute to protein secretion?

A

A: It processes and sorts proteins, preparing them for integration in the plasma membrane or for secretion via exocytosis.

105
Q

Q: Why is calcium storage in the smooth ER important?

A

A: Stored calcium ions are crucial for muscle contraction.

106
Q

Q: In what cellular processes is the smooth ER involved through detoxification?

A

A: Detoxifying drugs and harmful substances.

107
Q

Q: What structural feature distinguishes the rough ER from the smooth ER?

A

A: The rough ER has ribosomes on its surface, while the smooth ER does not.

108
Q

Q: What type of proteins does the rough ER primarily produce?

A

A: Membrane and cytosolic proteins.

109
Q

Q: Through which cellular organelle do proteins synthesized by the rough ER typically pass for further processing?

A

A: The Golgi complex.

110
Q

Q: Name two functions of the smooth ER related to lipid processing.

A

A: Synthesis of fatty acids and steroid hormones such as testosterone and estrogen.

111
Q

Q: How does the smooth ER contribute to glucose metabolism?

A

A: It processes glucose-6-phosphate, aiding in carbohydrate metabolism.

112
Q

Q: What specialized form of smooth ER is involved in muscle contraction?

A

A: The sarcoplasmic reticulum.

113
Q

Q: Which cellular process is smooth ER primarily not involved in due to its lack of ribosomes?

A

A: Protein synthesis.

114
Q

Q: How are the rough and smooth ER structures related to each other?

A

A: The smooth ER extends from the rough ER.

115
Q

Q: What are the structural components of the Golgi complex?

A

A: 3-20 flattened sacs called saccules.

116
Q

Q: How does the Golgi complex receive proteins, and from where?

A

A: Proteins are received at the entry (cis) face from the rough ER.

117
Q

Q: What happens to proteins in the intermediate saccules of the Golgi complex?

A

A: They are modified into glycoproteins and lipoproteins.

118
Q

Q: Name the three types of vesicles formed by the Golgi complex and their functions.

A

Secretory vesicles: Carry proteins outside the cell via exocytosis.
Membrane vesicles: Deliver new molecules to the plasma membrane.
Transport vesicles: Deliver proteins to organelles like lysosomes.

119
Q

Q: What are the primary functions of lysosomes?

A

Digesting substances from outside the cell, recycling worn-out organelles (autophagy), and potentially destroying the entire cell (autolysis).

120
Q

Q: How many different enzymes do lysosomes contain, and what is their purpose?

A

A: About 60 different digestive and hydrolytic enzymes for breaking down biological molecules.

121
Q

Q: What is autophagy, and how does it differ from autolysis?

A

A: Autophagy is the digestion of worn-out organelles, while autolysis is the destruction of the entire cell.

122
Q

Q: although similar in shape, how do peroxisomes differ from lysosomes?

A

A: They are smaller and contain enzymes that oxidize organic substances and detoxify harmful compounds.

123
Q

Q: What role does catalase play in peroxisomes?

A

A: Catalase breaks down hydrogen peroxide (H2O2) into water and oxygen, preventing cellular damage.

124
Q

Q: How can peroxisomes replicate?

A

A: By enlarging and dividing or forming anew from cellular components.

125
Q

Q: What is the primary function of proteasomes?

A

A: To degrade unneeded, damaged, or faulty proteins into small peptides and amino acids.

126
Q

Q: Where are proteasomes located?

A

A: In the cytosol and the nucleus.

127
Q

Q: How do proteasomes contribute to cellular homeostasis?

A

A: By regulating metabolic pathways through protein degradation, aiding in negative feedback.

128
Q

Q: What diseases are associated with malfunctioning proteasomes, and why is this significant?

A

A: Parkinson’s and Alzheimer’s, due to the accumulation of abnormal proteins; understanding this process may lead to treatment strategies.

129
Q

Q: What are the structures called that destroy unneeded, damaged, or faulty proteins by cutting them into smaller peptides?

A

A: Proteasomes

130
Q

Q: What happens to the smaller peptides produced by proteasomes?

A

A: They can be broken down by proteases into individual amino acids.

131
Q

Q: What is the final use of the amino acids that result from the breakdown of peptides?

A

A: They can be recycled into more functional, healthy proteins.

132
Q

Barrel-shaped structures that destroy unneeded, damaged, or faulty proteins by cutting long proteins into smaller peptides

A

Proteasomes

133
Q

Q: What is the primary function of mitochondria?

A

A: They generate most of the cell’s ATP through aerobic respiration.

134
Q

Q: How does the quantity of mitochondria vary in different types of cells?

A

A: The number of mitochondria depends on the cell’s activity level, with more present in active cells like muscle, liver, and kidney cells.

135
Q

Q: How does regular exercise affect mitochondria in muscle cells?

A

A: It increases the number of mitochondria, improving the efficiency of energy production.

136
Q

Q: What is the structure of mitochondria?

A

A: Mitochondria have an outer and inner membrane, with the inner membrane forming folds called cristae. They also have a central space known as the mitochondrial matrix.

137
Q

Q: What role do mitochondria play in apoptosis?

A

A: They initiate programmed cell death by releasing chemicals like cytochrome c, which triggers cell death pathways.

138
Q

Q: How do mitochondria replicate?

A

A: They can self-replicate during periods of increased energy demand or when cells divide. They also contain their own circular DNA.

139
Q

Q: How is mitochondrial DNA inherited?

A

A: It is inherited only from the mother, allowing for tracing of maternal lineage through mitochondrial DNA.

140
Q

Q: What structures are contained within the mitochondrial matrix?

A

A: It contains ribosomes and small, circular DNA molecules known as mitochondrial DNA (mtDNA).

141
Q

Q: What is the function of the cristae in mitochondria?

A

A: ATP is synthesized by enzymes on the cristae from energy extracted from carbohydrates, lipids, proteins, and alcohol.