Chapter 3

Question 1

Isolated colonies

Answer 1

Are separated from each other

Question 2

Colony morphology

Answer 2

Each microorganisms exhibits certain phenotype: SHAPE (round irregular pinpoint) MARGIN (undulate) ELEVATION (flat) TEXTURE (moist) PIGMENT PRODUCTION (shiny dull)

Question 3

Environmental factors include?

Answer 3

Oxygen, temperature, osmotic pressure, pH

Question 4

Oxygen types

Answer 4

OBLIGATE (strict) AEROBIC, requires presence of oxygen for growth, OBLIGATE (strict) ANAEROBIC, can’t grow in presence of oxygen, FACULTATIVE ANAEROBIC, grow in the presence or absence of oxygen but prefer oxygen, MICROAEROPHILLIC, requires oxygen, but at lower atmospheric concentrations (2-10%) greater than 10 toxic

Question 5

Anaerobic jar

Answer 5

This device will provide anaerobic environment free of O2 include: GAS PACK, PALLADIUM PACKETS, AND INDICATOR STRIPS

Question 6

Candle jar

Answer 6

Great for microaerophillic organisms, candle flame will consume oxygen in jar to about 2-5% produce elevated CO2 levels

Question 7

Fluid Thioglycollate Medium (FTM broth)

Answer 7

Determines growth characteristic of organism based on oxygen requirements. The reducing agent is THIOGLYCOLIC ACID creates anaerobic environment as tube gets deeper, METHYLENE BLUE/RESAZURIN redox indication turns pink in presence oxygen and colorless when oxygen absent