Chapter 2 - Microscopy Flashcards
What type of microscopy should you use to see a virus?
TEM (transmission)
What is resolution?
The ability to distinguish detail (use refraction to magnify sample)
What are some types of light microscopy?
Brightfield, fluorescence
Shorter wavelength means…
Better resolution
What is flourochrome?
Substances that absorb energy from short wavelengths of light and emit longer wavelengths
What happens in electron microscopy?
Electrons bounce off sample and the computer generates an image from the bounce analysis
What are some types of electron microscopy?
Scanning (SEM), transmission (TEM)
What do you use scanning EM?
To look at the surface of a sample
Why do you use transmission EM?
To look at subcellular structures (thin slices)
Which has greater magnification: scanning or transmission EM?
TEM
