Chapter 2 ~ Basic Components Of Living Systems Flashcards
What does cell theory state?
Both animals and plant tissues are composed of cells
Cells are the basic unit of life
Cells only developed from existing cells
Explain what light microscope are
They use light to magnify objects. They have two lenses and objective lens which is near the specimen this magnifies the sample and the eyepiece lens where the specimen is viewed from which further magnifies.X
What night microscopes used to view?
Whole cell or tissues as well as living cells
Explain electron microscopes
They use a beam of electrons
They have more detail on cell ultra structure
What is the two types of electron microscope?
Transmission electron microscope
Scanning electron microscope
Explain transmission electron microscope
Visualises individual organ using electron magnets to focus a beam of electrons at sample
Explain scanning electron microscope
It produces 3-D images of cells and organelles. It is done by emitting the beam of electrons towards the sample electrons which is used to build a image
Which one has higher resolution?
Transmission electron microscope
Which one has higher resolution?
Transmission electron microscope
Steps for calibration
- Place the stage micrometre on the stage and focused the lens so that you can clearly see the divisions.
- align the eyepiece graticule with the stage micrometre
- Each division of the stage micrometre is 0.1mm
- The gratitude is divided by a scale from 0 to 100
- Take away the stage micrometre and add the sample using the IPP gratitude to measure its size
What is the formula for magnification?
Size of image divided by size of object
What is the definition for magnification?
How many times larger the images than the actual size of the object being viewed
What is the definition of resolution and what type of microscope has a higher resolution?
Resolution is the ability to see individual objects as separate entities
Like microscopes have a much lower resolution so produce less detailed images
What are the four methods of preparing sample?
Dry mount
Wet mount
Squash slide
Smear slide
Explain dry mount
Solid specimens of viewed whole or cut into thin
And then the specimen is plates on the centre of a slide with a cover slip above
Explain wet mount
A specimen is put into the liquid and a cover slip is placed at an angle
Explain wet mount
A specimen is put into the liquid and a cover slip is placed at an angle
Explain squash slide
A wet mount is prepared and the lens tissue is pressed down a cover slip is placed and the sample is squashed between two microscope slides