Chapter 18 Flashcards
cells don’t replicate themselves, ____ replicates itself
DNA
Transforming principle: (by _____ and ___) rough and smooth strains in mice
Oswald Avery and Fredrick Griffith
chemists to solve mouse case: _____ and ____
____enters cells, not protein, so proved that DNA codes for _____, not proteins
Hershey and Chase
DNA
genes
what makes up a single nucleotide?
1 deoxyribose sugar, phosphate, base (ACTG)
What are the 4 nitrogenous bases?
A-adenine
T- thymine
C- cytosine
G- guanine
What is Chargaff’s rule of base pairing (example)
A=T and C=G
so if 17% T then also 17% A
What did Rosalind Franklin do?
took pics of DNA with x-rays
figured out double helix shape, and that bases are on the inside with phosphate and sugar on the outside
RNA is different from DNA in what ways?
- leaves the nucleus
- sugar backbone is ribose (not deoxyribose so it has all its O2)
- single stranded, not double helix
- can be tRNA, mRNA, or rRNA
- 3 bases code for an amino acid (codon- triplet coding)
If you have 2 codons, how many amino acids do you have? How many bases?
2 codons = 2 amino acids = 6 nitrogenous bases
What did Watson and Crick do?
produced a structural model of DNA with double helix and complimentary base pairs (took everyone else’s ideas)
____ makes up all of the DNA in the cell (all genes in body)
genome
Steps of DNA replication
- Initiation
- elongation
- termination
What are helicases and what do they do?
enzymes that cut/ unwind the DNA (in the first step of DNA replication- initiation), forming a replication bubble… if bubble keeps expanding = replication fork
What holds the unzipped DNA apart and serves as a template for new bases? (in initiation)
SSB- single strand binding proteins
____ makes up primer, which serves as a starting point, constructed by ____ _______
RNA, primase enzyme
what splices the okazaki fragments ?
DNA ligase
Transcription
splitting DNA and making a copy of RNA
Translation
proteins are made by bringing amino acids as described by the mRNA