Chapter 17

Question 1

This is not a living thing; is a nucleic acid which is enclosed by a protein capsule; and injects its own DNA or RNA to its host cell

Answer 1

Virus

Question 2

Enzymes produced by bacteria that cut DNA at specific base sequences

Answer 2

Restriction enzymes

Question 3

When the restriction enzyme cut the DNA this leaves bases unpaired and are called

Answer 3

Sticky

Question 4

What enzyme and comes in and joins the fragments left by the work of the restriction enzyme

Answer 4

DNA ligase

Question 5

What charge does DNA have

Answer 5

Negative

Question 6

This is a way to separate fragments of DNA by utilizing the negative charge of the DNA molecule most generally used in DNA fingerprinting

Answer 6

Gel electrophoresis

Question 7

How do the sizes of the DNA fragments move in gel electrophoresis

Answer 7

Small move further and faster

While large fragments move slower

Question 8

These are small circular pieces of DNA typically used to clone relatively small pieces of DNA

Answer 8

Plasmid vector

Question 9

When bacteria absorbs plasmids

Answer 9

Transformation

Question 10

This is a collection of lots of DNA fragments inserted into lots of plasmid vectors that are inserted into a lots of bacteria cells

Answer 10

DNA libraries

Question 11

Enzyme that forms by the retroviruses

Answer 11

Reverse transcriptase

Question 12

From the viral DNA reverse transcriptase makes cDNA name an example of this virus

Answer 12

HIV

Question 13

What is used to get clean DNA that is free of introns

Answer 13

Reverse transcriptase

Question 14

This is a quick an inexpensive way to make many copies of any DNA molecule

Answer 14

PCR

Question 15

Name steps in PCR to make copies of DNA

Answer 15

1: heated ; denatured
2: denatured strand are cooked and primers attach ( annealing)
3: primed DNA is heated with TAQ polymerase to extend primers