Chapter 16 - Analysing water for organic compounds

Question 1

what does HPLC stand for

Answer 1

high
performance
liquid
chromatography

Question 2

what is the mobile phase

Answer 2

the phase that moves through the stationary phase, pushing the sample through the column/area.

Question 3

what is the stationary phase

Answer 3

the phase that remains still throughout procedure

Question 4

define adsorption

Answer 4

molecules holding onto the outside surface of a material whether it be the mobile or stationary phase

Question 5

what happens with a non-polar stationary phase

Answer 5

a non-polar sample will adsorb more readily to it. this creates a longer retention time

Question 6

what type of data is retention time

Answer 6

qualitative data

Question 7

what type of data is peak area

Answer 7

quantitative data (as a calibration curve can be made to determine concentrations of molecules in the sample.)

Question 8

how can the name of molecules befound through HPLC

Answer 8

comparing two experiments in the exactly the same conditions. substances which have the same retention time are therefore the same substance

Question 9

what must a calibration curve have

Answer 9

labels (conc - peak/abosrbance)

accurately measured points

line of best fit that only goes from minimum point to maximum point

Question 10

what are the variables for a chromatogram

Answer 10

peak area on the y axis

retention time on the x axis