Chapter 13- RNA and Protein Synthesis Flashcards
Genes contain…
Coded DNA that give the cell instructions for building proteins
Scientists knew…
What genes were made of (DNA) but not how they worked
Three major differences in RNA (versus DNA)
The sugar is ribose not deoxyribose
Single stranded not double stranded
Contains uracil as fourth base not thymine
Three main types of RNA
mRNA(messenger)
rRNA(ribosomal)
tRNA(transfer)
Messenger RNA
Carries instructions from DNA out of the nucleus
Ribosomal RNA
RNA embedded in the ribosomes
Transfer RNA
Escorts an amino acid for attachment to growing proteins
RNA transcription
Converts DNA code into RNA so the rest of the cell can understand and use it
RNA polymerase
Synthesizes a single strand of RNA from a DNA template, similar to replication but now a pairs with u
Promoters
Act as sites for the RNA polymerase to start (DNA is a big molecule)
RNA editing (purpose)
Lots of genes were transcribed that weren’t needed
(RNA editing) introns
Parts of the code that are discarded
(RNA editing) exons
Parts of the code kept and spliced together in order
RNA editing (what it does)
Let’s different cells use the same segment of DNA to code for different proteins (different effects)
Proteins
Made of long chains of amino acids called polypeptides (20 different amino acids to choose from)
Nitrogenous bases in RNA
Three bases in a specific order to code for one amino acid
Codon
3 nitrogenous bases in mRNA
Anticodon
3 nitrogenous bases in tRNA (base pairs for the codon)
Reading codons
Must use chart on pg 367/311 of text to fully understand (will use example AGC)
Start with 1st nitrogenous base in the codon(a) find it in center of the chart
Moving away from the center of chart in a line from the 1st letter find the 2nd letter(g)
Moving still farther from the center find the 3rd letter (c)
The outermost part of the circle is the name of the amino acid the codon codes for
Methionine is the start code it is then read three bases at a time until the stop codon is reached
Translation
A ribosome attaches to an mRNA strand and reads the codon
It finds the tRNA anticodon with the attached amino acid
Attaches amino acid to the growing polypeptide chain
Detached tRNA
repeats for next codon
Central dogma of molecular biology
DNA–>RNA–>proteins
(Types of gene mutations) point mutations
Change one or a few nucleotides
Substitutions
Insertions
Deletions
(Types of gene mutations) frameshift mutations
Shift the sequence of codons
Chromosomal mutations
Changes the number or structure of chromosomes
Types of chromosomal mutations
Deletion
Duplications
Inversion
Translocation
Effects of mutations
Only mutations in DNA of sex cells are passed on to the next generation
Most mutations have no effect on the organism
Genetic disorders
Very harmful mutations
Helpful mutations
Pesticide resistance
Polyploidy- plants with extra sets of chromosomes (results in stronger larger plants)
Mutations
Caused by mutagens- chemical or physical agents in the environment