Chapter 12 - Key Concepts & Review Points

Question 1

Simple rate equations describe the progress of ________-order and ________-order reactions.

Answer 1

first

second

Question 2

The Michaelis-Menten equation relates the initial velocity of a reaction to the ____________ and the ____________ for a particular enzyme and substrate.

Answer 2

maximal reaction velocity

Michaelis constant

Question 3

An enzyme’s overall catalytic efficiency is expressed as ________.

Answer 3

Kcat/Km

Question 4

A Lineweaver-Burk plot can be used to present kinetic data and calculate values for ________ and ________.

Answer 4

Km

Vmax

Question 5

Bisubstrate reactions can occur by a(n) ___________ or ____________ sequential mechanism or by a(n) ____________ mechanism.

Answer 5

ordered
random
ping-pong

Question 6

Enzyme inhibitors interact ____________ or ____________ with an enzyme to alter its Km and/or Vmax values.

Answer 6

reversibly

irreversibly

Question 7

A(n) ____________ enzyme inhibitor binds to the enzyme’s active site and increases the apparent Km for the reaction.

Answer 7

competitive

Question 8

A(n) ____________ enzyme inhibitor affects catalytic activity such that both the apparent Km and Vmax decrease.

Answer 8

uncompetitive

Question 9

A(n) ____________ enzyme inhibitor alters both catalytic activity and substrate binding such that the apparent Vmax decreases and the apparent Km may increase or decrease.

Answer 9

mixed (non-competitive)